| 四季鹅C4B基因的克隆及其表达(英文) |
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| 引用本文： | 茆骏,邢光东,王根林.四季鹅C4B基因的克隆及其表达(英文)[J].农业科学与技术,2014(1):17-20. |
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| 作者姓名： | 茆骏 邢光东 王根林 |
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| 作者单位： | [1]南京农业大学动物科技学院,江苏南京210095 [2]江苏省农业科学院畜牧所,江苏南京210014 [3]郎溪县畜牧兽医局,安徽宣城242100 |
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| 基金项目： | 江苏省农业自主创新资金[CX（09）638] |
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| 摘 要： | 目的]克隆四季鹅的C4B基因,并检测其在鹅体内的表达情况。方法]应用RACEPCR的方法克隆C4B基因的cDNA序列,登录GenBank进行多物种蛋白氨基酸序列比对并构建进化树,以分析同源性。结果]四季鹅C4B与鸡、鹌鹑2种禽类的同源性较高;在成体鹅中C4B主要在肝、肺中呈现高水平表达,在附睾、输精管、脑、肾、睾丸、心、输卵管、小肠中呈低表达。结论]该试验应用荧光定量PCR的方法测定了C4B基因在正常状态下四季鹅不同组织、器官中mRNA的表达量,为快速诊断某些疾病提供依据。
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| 关 键 词： | 四季鹅 补体 C4B基因 克隆 |
Cloning and Expression of C4B Gene in Siji Goose |
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| Jun MAO,Guangdong XING,Genlin WANG.Cloning and Expression of C4B Gene in Siji Goose[J].Agricultural Science ＆ Technology,2014(1):17-20. |
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| Authors: | Jun MAO Guangdong XING Genlin WANG |
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| Institution: | 1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China 2. Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 3. Bureau of Animal Husbandry and Veterinary of Langxi Courty, Xuancheng 242100, China) |
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| Abstract: | Objective] This study aimed to clone C4B gene in Siji goose and detect its expression level in different tissues. Method] cDNA sequence of C4B gene was cloned with RACE-PCR method. Amino acid sequences in multiple species were aligned in GenBank, and a phylogenetic tree was constructed for homology analysis. Result] C4B gene in Siji goose shared relatively high homology with chicken and quail; Siji goose C4B gene was expressed highly in liver and lung of adult geese and expressed lowly in epididymis, seminiferous duct, brain, kidney, testis, heart, oviduct and smal intestine. Conclusion] In the present study, mRNA expression lev-el of C4B gene in different tissues and organs of Siji goose was determined by flu-orescence quantitative PCR, which provided basis for rapid diagnosis of specific an-imal diseases. |
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| Keywords: | Siji goose Complement C4B gene Cloning |
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