枳子、葛根花和苦参混合药物对小鼠急性酒精中毒的防治作用

目的]探讨中药枳子、葛根花和苦参混合药物对小鼠急性酒精中毒的防治作用。方法]采用酒精灌胃法建立小鼠急性酒精中毒模型，灌喂低、中、高3种剂量的药物，进行先药后酒的预防醉酒和先酒后药的治疗醉酒试验，统计醉酒小鼠数目和小鼠睡眠时间，并通过检测小鼠肝组织中的丙二醛（ MDA）、还原型谷胱甘肽（GSH）及血清甘油三酯（TG）的含量，研究药物对小鼠肝的保护作用。结果]药物的作用具有剂量依赖性，高剂量组药物较模型组能明显减少醉酒小鼠的数目，中剂量和高剂量药物组缩短醉酒小鼠的睡眠时间，并可以显著降低小鼠肝组织内丙二醛（ MDA）、血清甘油三酯（TG）的含量，低、中、高剂量组都能升高还原型谷胱甘肽（GSH）的含量。结论]枳子、葛根花和苦参具有预防和治疗醉酒的功效，而且对酒精引起的肝损伤具有一定的防治作用。

Prevention and Cure of Acute Alcohol Intoxica-tion in Mice by Administration of Compound of Japanese Raisintree Fruit,Lobed Kudzuvine Flower Bud and Lightyellow Sophora Root

Objective] We aimed to investigate the preventive and therapeutical effect of compound of traditional Chinese drugs （Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root） on acute alcohol intoxication in mice. Method] Acute alcohol intoxication was induced by administering alcohol to mice. Three different doses （low, middle and high） of compound of traditional Chinese drugs were administered to mice before and after administering alcohol respectively to investigate the preventive and therapeutical effect of drugs on acute alcohol intox-ication through doing statistical analysis about drunk mice and their sleeping time. The concentration of malondialdehyde （MDA）, reduced glutathione （GSH） and triglyc-erides （TG） in liver was also determined to investigate the protective effect of drugs on liver. Result] The efficacy of compound of traditional Chinese drugs on acute al-cohol intoxication was dose-dependent. High-dose administration decreased the number of drunk mice significantly compared with control group; middle- and high-dose administration reduced the sleeping time of drunk mice and the concentration of MDA and TG in liver tissue; three doses al increased the concentration of GSH. Conclusion] The compound of Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root had preventive and therapeutical effect on hangover, and it also had certain preventive and therapeutical effect on liver damage caused by alcohol.