| The Establishment of Immunochemistry Test Based on a Synthetic PeptideAntibody for the Detection of a Porcine Circovirus-Like Virus P1 |
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| 作者姓名： | Libin WEN Fengzhi WANG Kongwang HE Yanxiu NI Xuehan ZHANG Rongli GUO Bin LI Xiaomin WANG Zhengyu YU Aihua MAO Junming ZHOU Lixin LU Jianping XIE |
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| 基金项目： | Supported by National Natural Science Foundation of China （31272574, 30972184）. |
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| 摘 要： | Recently, a novel porcine circovirus-like virus P1 with a circular DNA genome of 0.648 kb was identified. P1 antigen was detected both in vitro and in vivo by synthetic peptide-derived polyclonal antibody-based immunochemistry. The designed peptides were synthesized by solid-phase technique, purified by high per-formance liquid chromatography, coupled to Keyhole limpet hemocyanin, and injected into rabbits to prepare polyclonal antibody. The emergence of positive cells revealed that synthetic peptide could elicit antibodies against P1 and viral protein could be synthesized. The polyclonal peptide antibodies described here was successfully ap-plied to immunochemical staining and proved helpful in diagnosing P1.
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| 关 键 词： | 猪圆环病毒 多克隆抗体 化学检测 合成肽 化学试验 免疫 液相色谱仪 病毒蛋白质 |
The Establishment of Immunochemistry Test Based on a Synthetic Peptide Antibody for the Detection of a Porcine Circovirus-Like Virus P1 |
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| Libin WEN,Fengzhi WANG,Kongwang HE,Yanxiu NI,Xuehan ZHANG,Rongli GUO,Bin LI,Xiaomin WANG,Zhengyu YU,Aihua MAO,Junming ZHOU,Lixin LU,Jianping XIE.The Establishment of Immunochemistry Test Based on a Synthetic PeptideAntibody for the Detection of a Porcine Circovirus-Like Virus P1[J].Agricultural Science ＆ Technology,2014(2):187-190. |
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| Authors: | Libin WEN Fengzhi WANG Kongwang HE Yanxiu NI Xuehan ZHANG Rongli GUO Bin LI Xiaomin WANG Zhengyu YU Aihua MAO Junming ZHOU Lixin LU Jianping XIE |
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| Institution: | Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Animal Diseases Diagnostic andImmunology, Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China |
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| Abstract: | Recently, a novel porcine circovirus-like virus P1 with a circular DNA genome of 0.648 kb was identified. P1 antigen was detected both in vitro and in vivo by synthetic peptide-derived polyclonal antibody-based immunochemistry. The designed peptides were synthesized by solid-phase technique, purified by high performance liquid chromatography, coupled to Keyhole limpet hemocyanin, and injected into rabbits to prepare polyclonal antibody. The emergence of positive cells revealed that synthetic peptide could elicit antibodies against P1 and viral protein could be synthesized. The polyclonal peptide antibodies described here was successfully applied to immunochemical staining and proved helpful in diagnosing P1. |
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| Keywords: | Porcine circovirus-like virus P1 Peptide antibo（Jy Immunochemistry test |
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