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小菜蛾化学感受蛋白突变体的构建(英文)
引用本文:刘晶晶,胡美英,任珍珍,李亚楠.小菜蛾化学感受蛋白突变体的构建(英文)[J].农业科学与技术,2012(1):177-180.
作者姓名:刘晶晶  胡美英  任珍珍  李亚楠
作者单位:华南农业大学昆虫毒理研究室
基金项目:Supported by Natural Science Foundation of Guangdong Province(9451064201003679);Guangdong Provincial Science and Technology Project(2009B020310005)~~
摘    要:目的]研究半胱氨酸(Cys58)对小菜蛾(Plutella xylostella)化学感受蛋白1(PlxyCSP1)与农药化合物结合特性的影响。方法]利用重叠延伸PCR法,将PlxyCSP1中Cys58突变成色氨酸(Trp58),获得突变体PlxyCSP1-M2。构建表达载体表达突变蛋白,并进行Western Blot检测。结果]构建了突变基因的原核蛋白表达载体pET32a-PlxyCSP1-M2,并表达35kDa融合蛋白结论]利用重叠延伸PCR法成功在原核系统中表达了PlxyC-SP1突变蛋白。

关 键 词:小菜蛾  化学感受蛋白1  原核表达

A Mutant of PlxyCSP1 Protein in Plutella xylostella
Jingjing LIU, Meiying HU, Zhenzhen REN, Yanan LI.A Mutant of PlxyCSP1 Protein in Plutella xylostella[J].Agricultural Science & Technology,2012(1):177-180.
Authors:Jingjing LIU  Meiying HU  Zhenzhen REN  Yanan LI
Institution:Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou 510642, China
Abstract:Objective] This study aimed to explore the effects of cysteine (Cys58) on the characteristics of PlxyCSP1 in P. lostella combined with pesticide compounds. Method] Cys58 of PlxyCSP1 was mutated into Trp58 by using overlap extension PCR method, and PlxyCSP1-M2 mutant was obtained. Expression vector was constructed and the protein was detected by western blot. Result] Expression vector pET32a-PlxyCSP1-M2 was constructed to express the 35kDa weight protein. Conclusion] PlxyCSP1 mutant protein has been expressed successfully in prokaryotic expression system.
Keywords:P  xylostella  PlxyCSP1  Prokaryotic expression system
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