| 地黄肌动蛋白基因片段的克隆与序列分析 |
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| 引用本文： | 孙鹏,郭玉海,祁建军,周莉丽,李先恩.地黄肌动蛋白基因片段的克隆与序列分析[J].农业科学与技术,2008,9(2):42-44. |
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| 作者姓名： | 孙鹏 郭玉海 祁建军 周莉丽 李先恩 |
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| 作者单位： | [1]中国农业大学农学与生物技术学院,北京100094 [2]中国医学科学院药用植物研究所,北京100094 |
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| 基金项目： | 国家自然科学基金，北京市自然科学基金 |
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| 摘 要： | 笔者从分子生物学基础较薄弱的药用植物地黄（Rehmannia glutinosa）中克隆分离肌动蛋白基因片段，并对所获得的地黄肌动蛋白基因序列进行分析。
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| 关 键 词： | 地黄 肌动蛋白基因片段 基因克隆 序列分析 药用植物 |
Cloning and Sequence Analysis of Actin Gene from Rehmannia glutinosa |
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| SUN Peng,GUO Yu-hai,QI Jian-jun,ZHOU Li-li,LI Xian-en.Cloning and Sequence Analysis of Actin Gene from Rehmannia glutinosa[J].Agricultural Science ＆ Technology,2008,9(2):42-44. |
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| Authors: | SUN Peng GUO Yu-hai QI Jian-jun ZHOU Li-li LI Xian-en |
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| Institution: | SUN Peng, GUOYu-hai, QI Jian-jun , ZHOU Li-li, LI Xian-en (1. College of Agronomy and Bioteehnology, China Agricultural University, Beijing 100094 ; 2. The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100094) |
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| Abstract: | Objective] The aim of this study is to clone and analyze the actin gene from Rehmannia glutina. Method] Degenerate primers were de-signed according to the conserved regions of actin sequences of Rehmannia glutinosa and its similar species, RT-PCR was next conducted to amplify the ac-tin gene from Rehmannia glutinosa. Result] The amplified fragment is 724 bp and correspondingly 240 amino acids. The BLAST results indicate that the homology between the amplified fragment and other higher plants for actin gene sequences and amino acid are more than 80% and 90%, respectively, sug-gesting that the amplified fragment is the actin gene ofRehmannia ghainosa. Conclusion] Phylogenetic analysis shows that the actin gene of Rehmannia glutinosa has an intimate genetic relationship with actin7 gene of Nicotiana tabacum. |
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| Keywords: | Actin Rehmannia glutinosa Sequence analysis Phylogenetic analysis |
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