多胺引发对黑麦草种子萌发期耐冷性的影响(英文)

目的]探讨多胺引发对黑麦草胚生理生化和种子发芽的影响。方法]以一年生黑麦草(钻石T和牧乐8000)为材料,种子经0.5mmol/L的腐胺(Put)、亚精胺(Spd)和精胺(Spm)引发24h后,在5℃下低温吸胀不同时间(12、24、36和48h),分析Put、Spd和Spm引发对一年生黑麦草种子吸胀中耐冷性和种子发芽能力的影响。结果]Put、Spd和Spm引发提高了胁迫期间种子胚内过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)活性,以及可溶性蛋白质含量,同时也显著提高发芽率、缩短平均发芽时间。低温吸胀48h后,与对照相比,Put、Spd和Spm处理分别提高一年生黑麦草种子的平均发芽率15.5%和12.0%,分别缩短平均发芽时间1.21和1.14d。在种子吸胀期间的耐冷性牧乐8000强于钻石T。总体而言,Put、Spd和Spm处理能提高一年生黑麦草种子吸胀期间的耐冷性,提高低温胁迫下种子发芽能力。结论]该研究结果为种子引发技术在一年生黑麦草生产上的应用提供了理论依据。

Effect of Polyamine Priming on Chilling Tolerance of Lolium perenne during Seed Imbibition

Objective] This study aimed to investigate the effect of polyamine priming on physiological and biochemical variations of Lolium perenne embryos and seed germination.Method] With annual Lolium perenne (Diamond T and Grazing-8000) as experimental materials,after priming with 0.5 mmol/L putrescine (Put),spermidine (Spd) and spermine (Spm) for 24 h and chilling imbibition at 5 ℃ for 12,24,36 and 48 h,the effect of Put,Spd and Spm priming on chilling tolerance and germination ability of annual Lolium perenne seeds during imbibition was investigated.Result] Put,Spd and Spm priming improved the activities of peroxidase (POD),catalase (CAT),superoxide dismutase (SOD) and ascorbate peroxidase (APX) and content of soluble protein content under low temperature stress,significantly increased the germination rate,and shortened the average germination duration.After chilling imbibition for 48 h,compared with the control,the average germination rate of annual Lolium perenne seeds was improved by 15.5% and 12.0% after Put,Spd and Spm priming,and the average germination duration was shortened by 1.21 and 1.14 d.During seed imbibition,the chilling tolerance of Grazing-8000 was stronger than that of Diamond T.Overall,Put,Spd and Spm treatment could increase the chilling tolerance of annual Lolium perenne seeds during imbibition,and improve the germination ability of seeds under low temperature stress.Conclusion] Results of this study provided theoretical basis for the application of seed priming technology in the production of annual ryegrass.