离体培养百里香精油的化学成分及抑菌活性研究(英文)

目的]比较分析普通百里香(Thymus vulgaris L.)组培苗、种子无菌苗和实生苗中精油的化学成分及相对含量,并对精油进行抑菌试验。方法]利用水蒸气蒸馏法提取精油,采用气质联用法并结合色谱峰面积归一化法分析比较其化学成分和相对百分含量,并用滤纸片法测定其抑菌活性。结果]3 种样品精油共分离出 54 种化学成分,其主要化学成分相同,均含有百里香酚、对伞花烃、松油烯等,只是相对含量略有差异;抑菌试验中,3 种样品精油对大肠杆菌和金黄色葡萄球菌都有明显的抑菌作用,其中对金黄色葡萄球菌的抑菌作用强于大肠杆菌。结论]采用离体培养方式生产百里香精油是可行的,其精油的主要化学成分以及抑菌活性基本保持不变。

Chemical Compositions and Antibacterial Activity of Essential Oil from Thymus Seedlings of Tissue Culture

Objective] This study aimed to compare and analyze the chemical compositions, the relative contents and the antibacterial activity of the essential oils extracted from the tissue culture seedlings, the aseptic seedlings and the common seedlings from seeds of Thymus vulgaris. Method] The essential oil was extracted by steam distillation; the chemical compositions and their relative contents were analyzed by gas chromatography-mass spectrometry (GC/MS) and peak area normalization method; and the antimicrobial activity was tested by filter paper method. Result] A total of 54 compositions were identified from these three samples, they had similar main compositions: thymol, m-Cymene, terpinene and so on, and these compositions were slightly different in contents. In addition, the essential oils from these three samples exhibited obvious antibacterial effect against Escherichia coli and Staphylococcus aureus, in which the antibacterial activity against S. aureus was stronger than against E. coli. Conclusion] It is possible to produce essential oil by tissue culture technology; the main compositions and antibacterial activity of the oils remained almost the same.