| 糖基转移酶基因sm-Ngt1对水稻株高的影响 |
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| 引用本文： | 王巍葳,谭艳平,王春台,刘学群.糖基转移酶基因sm-Ngt1对水稻株高的影响[J].农业科学与技术,2011(9):1279-1282. |
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| 作者姓名： | 王巍葳 谭艳平 王春台 刘学群 |
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| 作者单位： | 中南民族大学生命科学学院/生物技术国家民委重点实验室 |
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| 基金项目： | Supported by National Science Foundation of China(30871318);Key Research Project of Ministry of Education,PRC(210265)~~ |
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| 摘 要： | 目的]研究烟草中受甲基茉莉酸和水杨酸双重诱导的糖基转移酶基因sm-Ngt1过表达对水稻生长的影响。方法]构建sm-Ngt1的植物表达载体,采用农杆菌浸染法转化籼稻粤泰B,对获得的阳性转基因植株进行高度检测。结果]共获得117株阳性转sm-Ngt1植株。在成功转入sm-Ngt1基因后,水稻植株出现了不同程度的矮化,其中37%的转基因植株高度为71.4±9.8cm,27%的转基因植株高度为65.1±4.6cm,而未转基因的对照YTB的平均高度为130.0±4.3cm。结论]为进一步研究sm-Ngt1的功能奠定了基础。
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| 关 键 词： | 糖基转移酶 sm-Ngt1 水稻 矮化 |
Effects of Glycosyltransferase Gene sm-Ngt1 on the Plant Height of Rice |
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| WANG Wei-wei,TAN Yan-ping,WANG Chun-tai,LIU Xue-qun.Effects of Glycosyltransferase Gene sm-Ngt1 on the Plant Height of Rice[J].Agricultural Science ＆ Technology,2011(9):1279-1282. |
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| Authors: | WANG Wei-wei TAN Yan-ping WANG Chun-tai LIU Xue-qun |
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| Institution: | *Key Laboratory of Biotechnology,State Ethnic Affairs Commission,College of Life Science,South-Central University for Nationalities,Wuhan 430074 |
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| Abstract: | Objective] The aim was to study the effects of over-expressed sm-Ngt1,a glycosyltransferase gene induced by both methyl jasmonate and salicylic acid from tobacco,on the plant height of rice.Method] The binary expression vector of the sm-Ngt1 gene was constructed and transferred to matured embryo of indica rice YTB with the method of Agrobacterium infection.The height of the positive transgenic plants was measured.Result] 117 positive transgenic plants with sm-Ngt1 were obtained.The results showed that the rice plants dwarfed with different degrees after transferring the sm-Ngt1 gene,the height of 37% of transgenic plants is 71.4±9.8 cm,27% is 65.1±4.6 cm,and the average height of YTB(CK) is 130.0±4.3 cm.Conclusion] These results aid a foundafion for further study on function of sm-Ngt1. |
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| Keywords: | Glycosyltransferase sm-Ngt1 Rice Dwarf |
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