| 拟南芥CBF3基因克隆和植物表达载体的构建 |
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| 引用本文： | 张春平,束良佐,张启军,吕川根,蔡小宁.拟南芥CBF3基因克隆和植物表达载体的构建[J].农业科学与技术,2011(5):670-673. |
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| 作者姓名： | 张春平 束良佐 张启军 吕川根 蔡小宁 |
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| 作者单位： | 安徽农业大学;南京晓庄学院;江苏省农业科学院 |
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| 基金项目： | Supported by Cultivation for New Varieties of Genetically Modified Organisms Technology Projects(2008ZX08001-004);Key Projects of Nanjing Xiaozhuang University(2007NXY01);Natural ScienceFoundation for Jiangsu Province Universities(08KJD180011)~~ |
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| 摘 要： | 目的]克隆拟南芥CBF3基因并构建植物表达载体pCAMBIA1301-Rd29A-CBF3。方法]从模式植物拟南芥中克隆分离出转录因子CBF3与逆境诱导型启动子Rd29A的DNA序列,构建植物表达载体。结果]克隆得到的CBF3与GenBank数据库所公布序列比对发现,同源性达到100%,全长为750bp;克隆克隆得到的Rd29A与GenBank数据库公布序列比对发现,同源性达到100%,全长为1425bp。结论]以双元载体pCAMBIA1301为基础,植物表达载体pCAMBIA1301-Rd29A-CBF3被成功构建,对提高植物耐盐、耐旱和耐寒性有很大帮助。
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| 关 键 词： | CBF3 Rd29A 启动子 克隆 植物表达载体 |
Cloning of CBF3 Gene from Arabidopsis thaliana and Construction of Plant Expression Vector |
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| ZHANG Chun-ping,SHU Liang-zuo,ZHANG Qi-jun,LU Chuan-gen,CAI Xiao-ning.Cloning of CBF3 Gene from Arabidopsis thaliana and Construction of Plant Expression Vector[J].Agricultural Science ＆ Technology,2011(5):670-673. |
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| Authors: | ZHANG Chun-ping SHU Liang-zuo ZHANG Qi-jun LU Chuan-gen CAI Xiao-ning |
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| Institution: | 1.Anhui Agricultural University,Hefei 230036;2.Nanjing Xiaozhuang University,Nanjing 211171;3.Jiangsu Academy of Agricultural Sciences,Nanjing 210014 |
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| Abstract: | Objective] The aim was to clone CBF3 gene from Arabidopsis thaliana and construct plant expression vector pCAMBIA1301-Rd29A-CBF3.Method] CBF3 gene and stress-inducible promoter Rd29A were amplified from the genomic DNA of A.thaliana for the construction of plant expression vector.Result] Sequencing results showed that the cloned CBF3 gene had 750 bp,and showed 100% identity with the sequence published on GenBank.The promoter Rd29A had 1 425 bp,and showed 100% identity with the sequence published on GenBank.Conclusion] Based on the binary vector pCAMBIA1301,the plant expression vector pCAMBIA1301-Rd29A-CBF3 was constructed successfully,which could materially improve the salt resistance,drought-tolerance,cold resistance of plants. |
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| Keywords: | CBF3 Rd29A Promoter Clone Plant expression vector |
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