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施氮量及氮素形态对桑叶中1-脱氧野尻霉素含量的影响
引用本文:殷浩,佟万红,叶晶晶,孙波,施新琴,刘刚.施氮量及氮素形态对桑叶中1-脱氧野尻霉素含量的影响[J].农业科学与技术,2010,11(6):183-185.
作者姓名:殷浩  佟万红  叶晶晶  孙波  施新琴  刘刚
作者单位:殷浩(四川省蚕业研究所,四川南充,637000;四川企业科技开发公司,四川南地,637000;山东农业大学林学院,山东泰安,271018);佟万红,刘刚(四川省蚕业研究所,四川南充,637000);叶晶晶(山东农业大学林学院,山东泰安,271018);孙波(湖北省农业科学院经济作物研究所,湖北武汉,430064);施新琴(山东省蚕业研究所,山东烟台,264002) 
基金项目:Key Technology Innovation Project of Sichuan Province (2008NC00365).2008年四川省重点技术创新项目 
摘    要:目的]探讨施氮量及氮素形态对桑叶中1-脱氧脱尻霉素含量的影响,以期为提高桑叶中DNJ含量及其药用价值提供参考。方法]通过改变水培溶液中施氮量(NO3--N和NH4+-N)及氮素形态(NH4+/NO3-),研究其对桑叶主要药用成分1-脱氧野尻霉素(1-deoxynoji-mycin,DNJ)含量的影响。结果]随着2种氮素含量的增加,桑叶中DNJ均呈现出先升高后降低的趋势,且NO3--N比NH4+-N更利于DNJ的积累;随着NH4+/NO3-比的降低,桑叶中DNJ含量呈现出明显的先上升后下降的趋势,其中当比值为25/75时,含量达到最高。结论]适当的施氮量及NO--N/NH+-N比能有效提高桑叶中的DNJ含量。

关 键 词:桑叶  1-脱氧野尻霉素  施氮量  氮素形态

Effects of Nitrogen Amount and Nitrogen Form on 1-deoxynojimycin Content in Mulberry Leaf
YIN Hao,TONG Wan-hong,YE Jing-jing,SUN Bo,SHI Xin-qin,LIU Gang.Effects of Nitrogen Amount and Nitrogen Form on 1-deoxynojimycin Content in Mulberry Leaf[J].Agricultural Science & Technology,2010,11(6):183-185.
Authors:YIN Hao  TONG Wan-hong  YE Jing-jing  SUN Bo  SHI Xin-qin  LIU Gang
Institution:1.Sichuan Research Institute of Sericultural Sciences,Nanchong 637000;2.Sichuan Sericultural Technology Development Corporation,Nanchong 637000;3.College of Forestry,Shandong Agricultural University,Tai'an 271018;4.Institute of Industrial Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064;5.Shandong Research Institute of Sericultural Sciences,Yantai 264002
Abstract:Objective] Effects of nitrogen amount and nitrogen form on 1-deoxynojimycin(DNJ)content in mulberry leaf was explored in order to provide a reference for improving DNJ content in mulberry leaf and its medicinal value.Method] Effects of nitrogen amount(NO-3-N and NH+4-N)and nitrogen form(NO-3-N/NH+4-N)on DNJ content in mulberry leaf were studied by changing nitrogen amount and nitrogen form in water culture solution.Result] DNJ contents in mulberry leaf both increased at first and then decreased with the increase of nitrogen amount,furthermore compared with NH+4-N,NO-3-N was more beneficial to the accumulation of DNJ;DNJ content in mulberry leaf obviously increased at first and then decreased with the decrease of NO-3-N/NH+4-N,among them when its rate was 25/75,DNJ content reached the highest.Conclusion] Proper nitrogen amount and nitrogen form could effectively improve the DNJ content in mulberry leaf.
Keywords:Mulberry leaf  1-deoxynojimycin  Nitrogen amount  Nitrogen form
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