| 利用微卫星DNA标记分析贵州3个地方猪种的遗传多样性 |
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| 引用本文： | 郭宏宇,林家栋.利用微卫星DNA标记分析贵州3个地方猪种的遗传多样性[J].农业科学与技术,2009,10(1):77-80. |
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| 作者姓名： | 郭宏宇 林家栋 |
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| 作者单位： | 郭宏宇,GUO Hong-yu(山西农业大学动物科技学院,山西太谷,030801);林家栋,LIN Jia-dong(贵州大学动物科学学院,贵州贵阳,550025)
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| 摘 要： | 1材料与方法
1．1材料 采集3个地方猪种血液样品，共90份（表1）。采集个体品种特征明显，公母各半。
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| 关 键 词： | 微卫星DNA标记 地方猪种 遗传多样性 贵州 血液样品 品种特征 材料 采集 |
Genetic Diversity Analysis of Three Guizhou Local Pig Breeds Using Microsatellite DNA Markers |
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| GUO Hong-yu,LIN Jia-dong.Genetic Diversity Analysis of Three Guizhou Local Pig Breeds Using Microsatellite DNA Markers[J].Agricultural Science ＆ Technology,2009,10(1):77-80. |
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| Authors: | GUO Hong-yu LIN Jia-dong |
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| Institution: | 1. College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801 ; 2. College of Animal Science, Guizhou University, Guiyang 550025) |
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| Abstract: | Objective] The research aimed to provide the genetic basis for the protection, development and utilization of Guizhou local pig breeds. Method] From 27 pairs of porcine microsatellite primers recommended by Food and Agriculture Organization of the United Nations (FAO) and International Society for Animal Genetics (ISAG), six pairs (S0155, SW240, IGF1, SW951, SW857, SW24) were selected for microsatellite DNA detection of three Guizhou local pig breeds, including Nuogu Pig, Kele Pig and Guanling Pig. Subsequently, their genetic diversities were analyzed. Result] The three pig breeds were high polymorphic at the six microsatellite loci (PIC＞0.5). The Neis standard genetic distance of them was 0.206 3-0.481 5. The genetic distance between Nuogu Pig and Kele Pig was the closest, and that between Nuogu Pig and Guanling Pig was the furthest. Conclusion] The three Guizhou local pig breeds are in high genetic diversities. Nuogu Pig is a special type of Kele Pig, an excellent Chinese local pig breed. |
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| Keywords: | Guizhou local pig Microsatellite marker Genetic diversity Genetic relationship |
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