RP-HPLC法测定辅酶Q_（10）含量及其透光率的日变化分析（英文）

目的]建立高效液相色谱法测定辅酶Q10的含量。方法]采用反相高效液相色谱法（RP-HPLC）测定辅酶Q10的含量,并采用紫外分光光度计法对其透光率的日变化进行了分析。结果]辅酶Q10在RP-HPLC操作条件下分离度良好,在40～300μg/ml范围内与峰面积线性关系良好（r=0.9999）;最低检测限为0.4ng;平均回收率为97.44%（n=3）。RP-HPLC方法的系统适应性良好,回收率和精密度均满足分析要求。结论]该方法简便、准确、重现性好,可用于辅酶Q10的质量控制,但应注意避光操作。

Determination of Coenzyme Q10 Content by RP-HPLC and Daily Change of Transmittance of Co-Q10

Objective] The aim of this study was to establish a reversed phase high-performance liquid chromatography（RP-HPLC）method for the content determination of Co-Q10.Method] The RP-HPLC method was used to detect the Co-Q10,and the ultraviolet spectro-photometry was used to analyze the daily change of transmittance of Co-Q10.Result] By using RP-HPLC,Co-Q10 had a good linear relationship between 40-300 μg/ml（r=0.999 9）.The limit of detection was 0.4 ng and the average recovery was 97.44%（n=3）.The system suitability of HP-HPLC was good,and the average recovery and precision results could meet the needs of assay.Conclusion] This method was convenient,accurate and reproducible and could be used in quality control of Co-Q10.However,when it operates,light should be evaded.