萝卜离体小孢子培养中的胚胎发生及发育途径（英文）

目的]观察萝卜离体小孢子培养中的胚胎发生及发育途径。方法]以中国萝卜品种山东花叶心里美为试材,研究其离体小孢子培养中的细胞核分裂特征及发育途径。结果]离体小孢子培养过程中出现2种细胞核分裂特征的差异：①进行配子体发育的不均等分裂,即分裂形成2个大小不等的营养核和生殖核,该类小孢子形态与正常发育中双核期的小孢子相同;②进行孢子体发育的均等分裂,即形成2个大小相等的营养核。对小孢子发育进一步进行观察发现,均等分裂的细胞继续发育形成4细胞结构、多细胞团、球形胚、心形胚、鱼雷形胚及子叶形胚。结论]该研究结果为萝卜游离小孢子培养技术提供细胞学依据。

Embryogenesis and Development of Isolated Microspores in Raphanus sativus

Objective] The aim was to observe embryogenesis and development of isolated microspores of Raphanus sativus.Method] By using Chinese radish variety Shandong Huaye Xinlimei as the experimental materials,the caryocinesia features and development of isolated microspores in radish were studied.Result] Two types of cell division appeared in the isolated microspore：unequal division and equational division.The unequal divided microspores had two different size nucleuses,while the equational divided microspores had two same size nucleuses.The equational divided microspores developed to 4-cell structure,multi-cell structure,ball-shaped embryos,heart-shaped embryos,torpedo-stage embryos and cotyledon-stage embryos.Conclusion] This result will provide cytological basis for the isolated microspore culture of radish.