绿茶DNA提取方法及分子鉴定研究（英文）

目的]为绿茶产品品质鉴定提供依据。方法]以绿茶为原料,通过改进的CTAB方法来提取分离绿茶总DNA。并利用所得绿茶DNA采用ISSR分子标记对10个绿茶产品进行鉴定。结果]所采用改进的CTAB微量提取DNA法,可以得到高质量的绿茶总DNA,1.0g茶样DNA含量在101～498μg/g之间,平均249μg/g。采用ISSR分子标记检测结果表明,ISSR标记能有效地区别不同的绿茶产品。结论]该研究为进一步研究绿茶分子鉴定技术提取了参考依据。

DNA Extraction and Molecular Identification of Green Tea

Objective] The aim of this study was to provide reference for the quality identification of green tea.Method] Green Tea was used as materials,and its total DNA was extracted through improved CTAB method.And the obtained DNA was used to carry out identification on 10 varieties of green tea through ISSR molecular markers.Result] The high quality DNA from green tea could be obtained with new method,the DNA yield ranged from 101-498 μg/g tea sample for various green tea samples,and the average yield was 249 μg/g tea sample.The ISSR detection result showed that ISSR markers could effectively differentiate different varieties of green tea.Conclusion] The result had provided reference for the further study on molecular identification of green tea.