| 一种快速微量提取柑橘早期胚基因组DNA的方法（英文） |
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| 引用本文： | 范达,雷天刚,王军政,宋二玲,彭爱红,谭洪泉,王金萍,李政利,李凤龙,陈勇,陈善春.一种快速微量提取柑橘早期胚基因组DNA的方法（英文）[J].农业科学与技术,2010(11):95-97. |
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| 作者姓名： | 范达 雷天刚 王军政 宋二玲 彭爱红 谭洪泉 王金萍 李政利 李凤龙 陈勇 陈善春 |
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| 作者单位： | [1]西南大学园艺园林学院,重庆400716 [2]西南大学柑橘研究所,重庆400712 [3]国家柑橘品种改良中心,重庆400712 |
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| 基金项目： | 柑桔种苗纯度检测技术开发（国家科技支撑计划专项2007BAD47807-02）;柑桔分子育种与优异种质创新（重庆市重大科技专项CSTC,2007ABl040）. |
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| 摘 要： | 目的]建立一种快速微量提取柑橘基因组DNA的方法。方法]采用优化的CTAB微量提取法,以20.0,10.0,5.0,2.5mg的早期杂种胚为材料进行基因组DNA的提取,并对其DNA进行质量检测和SSR验证。结果]该方法简单易行,所需材料少,提取的DNA纯度较高,OD260nm/OD280nm在1.800～2.000,可满足SSR等以PCR扩增为基础的试验需要。结论]建立的方法可以用于快速微量提取柑橘基因组DNA。
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| 关 键 词： | 柑橘基因组 CTAB法 微量提取 PCR |
A Rapid Method for the Isolation of Small Amount DNA from Citrus Early Embryo |
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| FAN Da,LEI Tian-gang,WANG Jun-zheng,SONG Er-ling,PENG Ai-hong,TAN Hong-quan,WANG Jin-ping,LI Zheng-li,LI Feng-long,CHEN Yong,CHEN Shan-chun.A Rapid Method for the Isolation of Small Amount DNA from Citrus Early Embryo[J].Agricultural Science ＆ Technology,2010(11):95-97. |
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| Authors: | FAN Da LEI Tian-gang WANG Jun-zheng SONG Er-ling PENG Ai-hong TAN Hong-quan WANG Jin-ping LI Zheng-li LI Feng-long CHEN Yong CHEN Shan-chun |
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| Institution: | 1,2,3 1.College of Horticulture ＆ Landscape Architecture,Southwest University,Chongqing 400716;2.Citrus Research Institute,Southwest University,Chongqing 400712;3.National Citrus Cultivar Improvement Center of China,Chongqing 400712 |
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| Abstract: | Objective] The present study aimed to establish a rapid method for the isolation of small amount DNA from citrus.Method] By using the improved CTBA method,the genomic DNA was extracted respectively from 20,10,5 and 2.5 mg hybrid embryos of citrus,and then the DNA quality was detected and followed by SSR verification.Result] The method was very simple and rapid,which needed less materials.In addition,the isolated DNA showed good purity with the OD260/OD280 of 1.8-2.1,and could meet the requirement for PCR-based technology,such as SSR,etc..Conclusion] The method could be used for rapid extraction of small amount of genomic DNA from citrus. |
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| Keywords: | Citrus genome CTAB method Isolation of small amount DNA PCR |
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