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干旱胁迫对小麦萌发期POD、CAT活性的影响研究
引用本文:陈军,高贵珍,方雪梅,张兴桃,吕晓娇.干旱胁迫对小麦萌发期POD、CAT活性的影响研究[J].农业科学与技术,2013(11):1529-1531.
作者姓名:陈军  高贵珍  方雪梅  张兴桃  吕晓娇
作者单位:宿州学院化学与生命科学院
基金项目:Supported by Natural Science Fund of Education Department of Anhui Province(KJ2012B188);General Science and Research Program of Suzhou College(2011yyb13)~~
摘    要:目的]研究干旱胁迫对小麦萌发期POD、CAT活性的变化。方法]选取豫农51、周麦22、济麦19、淮麦22等4种小麦品种,用不同浓度 PEG6000模拟不同干旱程度,分别测定其 POD、CAT酶活性。结果]小麦萌发期在0-120 h 期间,5%、15% PEG6000胁迫下POD、CAT活性均呈逐渐上升趋势,但15%PEG6000胁迫下 POD、CAT 活性比5%PEG6000胁迫下其活性增幅大;25%、35%PEG6000胁迫下 POD、CAT活性均呈先上升后下降的趋势。胁迫时间对小麦萌发过程POD、CAT活性有显著影响。结论]该研究可为小麦抗旱育种提供理论参考。

关 键 词:小麦  萌发期  过氧化物酶活性  过氧化氧酶活性

Effects of Drought Stress on Activity of POD and CAT in Wheat during Generation Period
Institution:Jun CHEN;Guizhen GAO;Xuemei FANG;Xingtao ZHANG;Xiaojiao LU;Department of Chemistry and Life Sciences,Suzhou College;
Abstract:Objective] This study aimed to investigate the changes in activity of POD and CAT in wheat during generation period under drought stress. Method] Four wheat cultivars, including Yunong 51, Zhoumai 22, Jimai 19 and Huaimai 22, were selected as experimental materials and cultured under drought stress simulated by different concentrations of PEG6000, to determine the activity of POD and CAT. Result] POD and CAT activity in wheat during generation period increased gradual y under stress of 5% and 15% PEG6000 from 0 to 120 h; POD and CAT activity in wheat under stress of 15% PEG6000 increased more compared with that under stress of 5% PEG6000; POD and CAT activity in wheat under stress of 25% and 35% PEG6000 increased first and then decreased. Stress duration significantly af-fected the activity of POD and CAT in wheat during generation period. Conclusion] This study provided theoretical references for breeding drought-tolerant wheat.
Keywords:Wheat  Germination period  POD activity  -CAT activity
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