荧光茧色判性家蚕黄酮合酶Ⅰ（BmFNS Ⅰ）基因在家蚕组织内的表达差异

采用荧光定量PCR分别检测不同品种荧光判性家蚕雌、雄家蚕的中肠、丝腺和脂肪体中的黄酮合酶Ⅰ（BmFNS Ⅰ）基因的表达。结果表明,家蚕BmFNSI基因在各品种荧光判性家蚕中肠中的相对表达量最高,其次是脂肪体,在丝腺中的表达量比较低,说明BmFNSI基因在对家蚕食物桑叶内的黄酮类化合物的合成代谢中起重要作用;但是在各品种荧光判性家蚕的雌、雄中肠中的表达并无明显差异,说明荧光判性家蚕雌、雄蚕在对桑叶内的黄酮类化合物的合成代谢途径上不存在差异,至少在FNSI基因所调节的黄酮类化合物的合成代谢上不存在差异。

Expression Differences of Flavonoid Synthase Ⅰ（BmFNS Ⅰ）in the Tissues of Bombyx Mori with Sex-identified Fluorescent Cocoon Color

Expression differences of flavonoid synthase I（BmFNS I）gene in the midgut,silk gland and fat body of the male and female Bombyx Mori with sex-identified fluorescent cocoon color were detected by fluorescence quantitative PCR.The result showed that the relative expression level of BmFNS I gene in midgut of Bombyx Mori was the highest,followed by that in fat body,and the expression level in silk gland was the lowest,indicating that BmFNS I gene played an important role in flavonoids anabolism in mulberry leaves,which was the food of silkworm.However,there was no significant difference in BmFNS I gene expression level between midguts of male and female Bombyx Mori species with sex-identified fluorescent cocoon color,suggesting that there was no difference in flavonoids anabolism of mulberry leaves between the male and female Bombyx Mori with sex-identified fluorescent cocoon color,at least no difference in flavonoids anabolism regulated by FSN I gene.