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基于体细胞组织培养的甘蓝型油菜核三系保持系绵7MB-1亲本繁殖与F1制种
引用本文:蒙大庆,袁代斌,张跃非,郭子荣,胥岚,李芝凡,蒲定福,汤天泽,贺启川,陈军. 基于体细胞组织培养的甘蓝型油菜核三系保持系绵7MB-1亲本繁殖与F1制种[J]. 农业科学与技术, 2009, 10(1): 22-25,114
作者姓名:蒙大庆  袁代斌  张跃非  郭子荣  胥岚  李芝凡  蒲定福  汤天泽  贺启川  陈军
作者单位:四川绵阳市农业科学研究所,四川绵阳,621023  
基金项目:四川省农作物育种攻关,四川省联合育种攻关经费资助项目 
摘    要:1材料与方法1.1材料 核三系临保系:绵7MB-1;核两系不育系:绵7AB-4—1;恢复系:绵恢9613及品93-496(引自四川省农业科学院)。诱导培养基:B5+谷氨酰胺2mg/L+水解乳蛋白50mg/L+6-BA2mg/L+2T 1mg/L。增殖培养基:MS+6-BA 1 mg/L+NAA 0.1mg/L。

关 键 词:体细胞组织培养  甘蓝型油菜  亲本繁殖  三系  保持系  四川省农业科学院  制种  两系不育系

Reproduction of the Three-line Genic Male Sterile Line Parent Mian 7MB-1 (Brasscia Napus L.) and Seed Production of F1 Based on Somatic Tissue Culture
MENG Da-qing,YUAN Dai-bin,ZHANG Yue-fei,GUO Zi-rong,XU Lan,LI Zhi-fan,PU Ding-fu,TANG Tian-ze,HE Qi-chuan,CHEN Jun. Reproduction of the Three-line Genic Male Sterile Line Parent Mian 7MB-1 (Brasscia Napus L.) and Seed Production of F1 Based on Somatic Tissue Culture[J]. Agricultural Science & Technology, 2009, 10(1): 22-25,114
Authors:MENG Da-qing  YUAN Dai-bin  ZHANG Yue-fei  GUO Zi-rong  XU Lan  LI Zhi-fan  PU Ding-fu  TANG Tian-ze  HE Qi-chuan  CHEN Jun
Affiliation:( Mianyang Institute of Agricultural Sciences, Mianyang 621023)
Abstract:[Objective] The aim was to study the reproduction of the three-line genic male sterile (GMS) line parent Mian 7MB-1 (B.Napus L.) and the seed production of F1 through somatic tissue culture. [Method] Through hybridization, a new breeding material Mian 7MB-1 in three-line genic temporary maintainer line propagated by tissue culture was used to improve the sterile plant rate of rapeseed in dual-purpose recessive GMS line, such as Mian 7AB type, S45AB type, and etc. And then the variety comparative test was performed. [Result] In order to avoid some fertility restoration phenomena occurring during the process of self-reproduction, Mian 7AB was propagated in bulk with somatic tissue culture of temporary maintainer line plant stem. The propagated temporary maintainer line seedlings were applied to the breeding and seed production of net room male sterile line parent, promoting the sterile plant rate of the male sterile line parent to 91.7%-93.5%. The male sterile line parents per hectare were enough for the seed production of hybrid F1 in 7 500-15 000 hm2. [Conclusion] Compared with the original dual-purpose GMS line, the seed production ultilizing male sterile line with high sterile plant rate greatly reduced the labor, significantly improved the seed yield, ensuring the seed quality and forming a perfect breeding and seed production system.
Keywords:B.Napus L.  Tissue culture  Three-line GMS  Breeding and seed production
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