| 藏獒血液蛋白多态性研究 |
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| 引用本文： | 兰小平,郭宪,陈永昌,鄢珣,崔泰保.藏獒血液蛋白多态性研究[J].农业科学与技术,2009,10(4):50-54. |
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| 作者姓名： | 兰小平 郭宪 陈永昌 鄢珣 崔泰保 |
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| 作者单位： | 兰小平(天水师范学院生命科学与化学学院,甘肃天水,741000);郭宪(中国农业科学院兰州畜牧与兽药研究所,甘肃兰州,730050);陈永昌(中国科学院昆明动物研究所,云南昆明,650223);鄢珣,崔泰保(甘肃农业大学,甘肃兰州,730070) |
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| 基金项目： | Foundation of Gansu Technology Committee，Youth Foundation of Tianshui Normal University (X4-25).省科技厅基金资助项目，天水师范学院青年基金资助项目 |
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| 摘 要： | 1材料与方法
1．1材料共采集血样103份，其中，44份河曲藏獒（HTM），42份青海藏獒（QTM），5份青海藏狮犬（QTS）以及12份青海土种犬（NAD）。均采集后肢静脉肝素钠抗凝血5—10ml，24h内带回实验室，处理后置低温冰箱（-40℃）冻存备用。
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| 关 键 词： | 河曲藏獒 血液蛋白多态性 低温冰箱 青海 抗凝血 肝素钠 实验室 材料 |
Study on Polymorphisms in the Blood Protein of Tibetan Mastiff |
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| LAN Xiao-ping,GUO Xian,CHEN Yong-chang,YAN Xun,ICUI Tai-bao.Study on Polymorphisms in the Blood Protein of Tibetan Mastiff[J].Agricultural Science ＆ Technology,2009,10(4):50-54. |
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| Authors: | LAN Xiao-ping GUO Xian CHEN Yong-chang YAN Xun ICUI Tai-bao |
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| Institution: | 1. School of Life Science and Chemistry, Tianshui Normal University, Tianshui 741000; 2. Lanzhou Institute of Animal Science ＆ Veterinary Medicine, The Chinese Academy of Agricultural Sciences, Lanzhou 730050; 3. Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223; 4. Gansu Agricultural University, Lanzhou 730070) |
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| Abstract: | Objective] The aim was to study the protein polymorphism in the blood of Tibetan Mastiff, and provide some theoretical basis for resource protection and reasonable development and utilization of Tibetan Mastiff varieties. Method] A total of 103 blood samples were taken from four populations of Hequ Tibetan Mastiff, Qinhai Tibetan Mastiff, Tibetan Spaniel and native dogs of Qinghai. Seven blood protein Iocus（Tf, Po, Sα2, Hb, AIb, Pr and Amy）were investigated by using vertical polyacrylamide gel electrophoresis with discontinuous buffer system. Then the genetic variation during different populations was analyzed. Result] Genetic variations were observed in Tf, Sα2 and Po in four populations, others were not polymorphic. There were three alleles at the locus of Tf and Po, two alleles at the loci of Sα2. Effective number of alleles and Nei＇s average expected heterozygosity were 1. 532 4 and 0.230 3 relatively, all higher in Tibetan Mastiff than other populations. Conclusion] Protein locus in blood of Tibetan Mastiff existed in genetic variation. |
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| Keywords: | Tibetan Mastiff Blood protein polymorphism Effective number of alleles Nei's average expected heterozygosity |
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