| 香蕉MaPRMT1基因的分离及表达分析(英文) |
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| 引用本文： | 刘凡,张建斌,贾彩红,杨景豪,徐碧玉,金志强.香蕉MaPRMT1基因的分离及表达分析(英文)[J].农业科学与技术,2008,9(3):70-74. |
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| 作者姓名： | 刘凡 张建斌 贾彩红 杨景豪 徐碧玉 金志强 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所;海南大学农学院 |
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| 基金项目： | 公益性行业(农业)科研专项基金 |
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| 摘 要： | Objective] The aim of experiment was to lay molecular foundation for studying maturity mechanism of banana after harvest. Method] The combined method of suppressing subtractive hybridization and cDNA micro-array were used to obtain cDNA segment of one PRMT gene in banana and the whole cDNA sequence of the gene was cloned.The bioinformatics analysis was operated on it,in addition, the expression profile analysis was conducted in different organs and different mature periods of banana.Result] The whole length of cDNA in MaPRMT1 was 1 158 bp and possessed a complete open reading frame,which could encode 385 amino acids.It had high homology with PRMT in plant,containing one Methyltransf1 domain.The MaPRMT1 gene was expressed in root,stem,leaf and fruit of banana and the expression levels in stem and leaf were relatively high.As the increase of days after harvest,the expression level declined gradually,however it reached maximum when ethylene release was biggest,then it declined.Conclusion] MaPRMT1 belonged to the first kind of arginine methyltransferase and it was expressed differently in different organs and fruits at different mature periods.
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Isolation and Expression Analysis of MaPRMT1 Gene in Banana |
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| LIU Fan,ZHANG Jian-bin,JIA Cai-hong,YANG Jing-hao,XU Bi-yu,JIN Zhi-qiang.Isolation and Expression Analysis of MaPRMT1 Gene in Banana[J].Agricultural Science ＆ Technology,2008,9(3):70-74. |
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| Authors: | LIU Fan ZHANG Jian-bin JIA Cai-hong YANG Jing-hao XU Bi-yu JIN Zhi-qiang |
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| Institution: | 1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science,Haikou 571101; 2. College of Agriculture,Hainan University,Danzhou 571737 |
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| Abstract: | Objective] The aim of experiment was to lay molecular foundation for studying maturity mechanism of banana after harvest. Method] The combined method of suppressing subtractive hybridization and cDNA micro-array were used to obtain cDNA segment of one PRMT gene in banana and the whole cDNA sequence of the gene was cloned.The bioinformatics analysis was operated on it,in addition, the expression profile analysis was conducted in different organs and different mature periods of banana.Result] The whole length of cDNA in MaPRMT1 was 1 158 bp and possessed a complete open reading frame,which could encode 385 amino acids.It had high homology with PRMT in plant,containing one Methyltransf_1 domain.The MaPRMT1 gene was expressed in root,stem,leaf and fruit of banana and the expression levels in stem and leaf were relatively high.As the increase of days after harvest,the expression level declined gradually,however it reached maximum when ethylene release was biggest,then it declined.Conclusion] MaPRMT1 belonged to the first kind of arginine methyltransferase and it was expressed differently in different organs and fruits at different mature periods. |
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