新型甘蓝型油菜核不育材料绵7AB-4-2农艺性状鉴定及分子检测

四川省绵阳市农科所1991年用甘蓝型油菜双高隐性核不育系绵1AB-1作母本，品872—161—134作父本进行转育，F2代出现不育株进行成对兄妹交，经多代选择，培育出隐性核不育系绵7AB-4（双低）。以其不育系的不育性状为选择目标，从中分离纯化出2个农艺性状无明显差异、育性不同的株系，分别定为绵7AB-4-1和绵7AB-4-2，前者兄妹交和可育株自交的不育率分别为50％和25％，后者分别达到80％和60％。笔者对新材料绵7AB-4-2与其姊妹系绵7AB-4—1的农艺性状和SSR引物多态性进行研究，为其进行分子标记、克隆及进一步利用奠定基础。

Molecular Markers and Agronomic Traits of a New Kind of Genic Male Sterile Material Mian 7AB-4-2 in Brassica napus L.

Objective] The study was to investigate the agronomic traits and breeding characteristics of genic male sterile material Mian 7AB-4-2 in Brassica napus. Method] The differences in agronomic traits and polymorphisms in SSR markers, between the genic male sterile material Mian 7AB-4-2 in Brassica napus and its sisterly line Mian 7AB-4-1 were investigated by hybridization and molecular identification; and the percentage of sterile individuals of Mian 7AB-4-2 and of the hybrids with its sisterly line Mian 7AB-4-1 from test cross and back cross were also studied. Result] Mian 7AB-4-2 was not significantly different in agronomic traits from its sisterly line Mian 7AB-4-1 at 0.05 probability level. The percentages of sterile individuals in the pollinated fertile Mian 7AB-4-2 plants were over 60%, and that in its sisterly line Mian 7AB-4-1 was about 25%. In test crosses with other nine sterile lines, Mian 7AB-4-1 kept the percentage of sterile individuals of sterile lines over 90%, and the percentage of sterile individuals from back cross over 80%. With regard to molecular markers, Mian 7AB-4-2 and its sisterly line Mian 7AB-4-1 were different in the band number from SSR primers a2 and E10. Conclusion] The results indicate that Mian 7AB-4-2 is helpful for rapeseed breeding, quickening the application of new materials in field breeding.