13/17罗伯逊易位猪CMYA3基因多态性分析（摘要）（英文）

目的]对13/17罗伯逊易位杂合子猪2n=36,rob（13;17）]互交后代中的148个体进行CMYA3基因的多态性分析。方法]采用PCR-RFLP方法分析CMYA3基因在13/17罗伯逊易位猪群中的多态性。用酚-氯仿抽提法从猪耳组织中提取基因组DNA。以基因组DNA为模板进行PCR扩增,反应体系为25μl：dNTPs 2μl,上下游引物各0.5μl,Taq酶1 U,模板DNA1μl,用ddH2O补足至25μl。PCR反应条件为：94℃预变性5 min;94℃变性30 s,53℃退火45 s,72℃延伸30 s,共35个循环;72℃延伸10 min。PCR产物经PCR纯化试剂盒纯化,限制性内切酶Bsh1236 I 37℃酶切过夜后,1%琼脂糖凝胶电泳,检测酶切结果。结果]通过PCR扩增所有个体均能获得507 bp的目的片段,PCR产物经限制性内切酶Bsh1236I酶切,琼脂糖凝胶电泳结果表明该基因在此群体中具有A和B 2个等位基因,基因频率分别为0.699和0.301,具有AA、AB和BB 3种基因型,基因型频率分别为0.615、0.169和0.216。因此,在该试验猪群中,A为优势等位基因,AA基因型频率最大。结论]该研究为13/17罗伯逊易位猪的分子育种和标记辅助选择提供了理论依据。

Polymorphisms of CMYA3 Gene in 13/17 Robertsonian Translocation Pigs

Objective] The aim was to study the polymorphism of CMYA3 gene in the 148 pigs of hybrid offspring of 13/17 Robertsonian translocation pigs 2n = 37,rob （13;17）] intercrossing.Method] PCR-RFLP method was adopted.Result] A 507 bp fragment of CMYA3 gene was obtained by PCR amplification,and then amplification product by using restriction nuclease Bsh1236Ⅰ was detected by agarose gel electrophoresis.As a result,both alleles （A and B） of the loci were found in the population.The frequencies of allele A and B were 0.699 and 0.301.The genotype frequencies of AA,AB and BB were 0.615,0.169 and 0.216.The frequencies of allele A and genotype AA were significantly higher than allele B and genotype BB in populations.Conclusion] The study will provide theoretical basis for molecular breeding and marker-assisted selection of 13/17 Robertsonian translocation pigs.