新疆泥火山土壤宏基因组DNA的提取方法研究（摘要）

目的]寻找一种有效的泥火山土壤宏基因组DNA的提取方法。方法]分别采用CTAB法、SDS加酶法、实验室改进法、试剂盒法提取泥火山土壤宏基因组,比较4种方法的提取效果。结果]试剂盒法的提取效率最高、纯度最好;实验室改进法次之;SDS加酶法提取量最多。实验室改进法获得的DNA,稀释10倍可直接用于PCR扩增。结论]从经济和DNA质量的角度考虑,实验室改进法可作为新疆泥火山土壤宏基因组DNA提取最有效的方法。

Studies on the Extraction Methods of Metagenomic DNA from Mud Volcano in Xinjiang

Objective]To seek one effective extraction method of metagenomic DNA from mud volcano.Method]The metagenomic DNA from mud volcano was extracted by CTAB extraction method,SDS-enzyme method,improved method,reagent kit method.The extraction of four kinds of methods were compared.Result]The extracted rate in reagent sets method was the highest,next was improved method,the extracted quantity in SDS-enzyme method was maximum.DNA extracted by the improved method was diluted ten times for PCR.Conclusion]Considering economy and purity,the improved method can be used as one effective extraction method of metagenomic DNA from mud volcano.