转基因抗虫水稻中BT蛋白表达量的研究(英文)

对转基因抗虫水稻中Bt蛋白表达量进行研究。方法]应用酶联免疫吸附测定法(ELISA)定量检测转基因抗虫水稻生同生长期不同部位的Bt蛋自表达量。结果]转基因水稻灌浆期不同组织中Bt蛋白表达绝对含量的高低顺序为:叶片>未成熟种子及颖壳>根>茎杆;在水稻不同的生长发育(分蘖期、抽穗期和灌浆期)阶段,转基因Bt水稻中Bt蛋白的含量有一些变化;一般在水稻生长后期Bt蛋白的浓度有所下降,但幅度不大,对其抗性不会造成太大影响。结论]该试验对田间害虫的防治以及转基因水稻的育种都具有重要意义。

Expression of Bt Protein in Transgenic Pest-resistant Rice

Objective] The aim of this study was to study on expression of Bt protein in transgenic pest-resistant rice.Method] Enzyme-linked immunosorbent assay(ELISA) was used to measure Bt protein expression in different tissues of transgenic pest-resistant rice at same growth stage.Result] Absolute content of Bt protein from high to low was as follows:leaves > immature seeds and glumes > roots > stems in different tissues of transgenic rice in grain-filling stage;Bt protein content of transgenic rice changed a little in different growth stages(including tillering stage,booting stage,and grain-filling stage);in general,its level declined a little in later growth stage,but the resistibility would not be influenced significantly.Conclusion] The experiment is significant for pest prevention and transgenic rice breeding.