水稻miRNA应答低能N^＋束辐照的基因芯片分析

目的]探讨水稻应答N^＋束辐照机制中相关miRNA的表达差异。方法]各选取3组N^＋束辐照处理水稻种子和未处理的水稻种子,提取其在30℃发芽96h幼苗总RNA,采用Agilent单通道水稻基因表达谱芯片筛选2组水稻幼苗差异表达基因。芯片中含有miRNA探针510个,分析其在样品间表达差异2倍左右作为域值范围。结果]辐照组与对照组相比表达差异显著的miRNA分子有14个,且均为下调。结论]水稻N^＋束辐照组和对照组中存在差异表达的miRNA分子,可能调控某些基因的表达来应答离子辐照,从而表现出与对照不一样的生理生化和表型差异。

Gene Chip Analysis for Rice MicroRNA Response to Low Energy N+ Beam Radiation

Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N＋ beam radiation in rice.Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds were selected respectively,and then the total RNA of seedlings after 96 h of germination was extracted at 30 ℃.Agilent gene chip was used to screen the differentially expressed genes of two groups of rice seedlings.The chip contained 510 miRNA probes;and about two times of expression differences between two samples were considered as the threshold range.Result]14 miRNA molecules showed significant expression differences between the irradiated group and the control group,and all of them were decreased.Conclusion]The miRNAs showed expression differences between irradiated group and the control group,which might regulate the expression of certain genes to respond to ion irradiation,thus producing physiological,biochemical and phenotypic differences.