暗紫红毛菜ITS序列的测定与分析

目的]对暗紫红毛菜rDNA内转录间隔区（ITS区）进行序列测定,为其系统发育研究提供新资料。方法]以产于山西娘子关泉的一株暗紫红毛菜为材料,提取DNA,设计引物,进行PCR扩增,进而测定其ITS区基因序列。结果]暗紫红毛菜与同科的少精紫菜ITS区同源性为75%,与条斑紫菜ITS区同源性为79%。结论]ITS区序列进化速率相对较快,种类和地理分布的差异是其序列差异产生的原因。

Sequencing and Analysis of ITS Sequences of Bangia atropurpurea

Object]To providing new data for phylogenesis of Bangia atropurpurea by the sequencing of internal transcribed spacer（ITS region）of rDNA.Method]B.atropurpurea was collected from Niangziguan Spring in Shanxi,the DNA was extracted and the primers were designed for PCR amplification so as to obtain ITS gene sequence.Result]The homology of ITS region between B.atropurpurea and Porphyra oligospermatangia was 75%,which was 79% between B.atropurpurea and P.yezoensis.Conclusion]Compared with other genes,the ITS sequence had a greater evolution rate.The differences in classification and distribution resulted in the sequence diversity.