| 枸杞花药培养的初步研究(英文) |
| |
| 引用本文： | 段丽君,周军,曹有龙.枸杞花药培养的初步研究(英文)[J].农业科学与技术,2008,9(5):92-95. |
| |
| 作者姓名： | 段丽君 周军 曹有龙 |
| |
| 作者单位： | 宁夏农林科学院枸杞工程技术研究中心;西南林学院资源学院 |
| |
| 基金项目： | 国家科技成果转化项目
，
国家自然科学基金
|
| |
| 摘 要： | To investigate the culture technique in anther of Chinese wolfberry,we optimized the culture medium(including hormone combination)and culture conditions.The results showed that calluses were induced from all the six tested Chinese wolfberry materials,but the induction rate of callus varied toward the materials with different genotypes.When the experimental materials were cultured on medium appended with 2,4-D 1.0 mg/L and KT 1.0 mg/L under dark,the callus induction rate reached 20.0 % in this study,and this hormone combination should be the optimum for anther culture of Chinese wolfberry.With MS appended with 6-BA 0.5 mg/L and NAA 0.1 mg/L as differentiation medium and that appended with NAA 0.1 mg/L,the plants could be yielded in 20 days.
|
| 关 键 词： | Lycium barbarum L. Anther culture Callus tissue Regenerated plantlets |
Preliminary Study on the Anther Culture in Lycium barbarum L. |
| |
| DUAN Li-jun,ZHOU Jun,CAO You-long.Preliminary Study on the Anther Culture in Lycium barbarum L.[J].Agricultural Science ＆ Technology,2008,9(5):92-95. |
| |
| Authors: | DUAN Li-jun ZHOU Jun CAO You-long |
| |
| Institution: | 1,21.Engineering Technology Research Center of Chinese Wolfberry,Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan 750002;2.College of Resources,Southwest Forestry University,Kunming 650224 |
| |
| Abstract: | To investigate the culture technique in anther of Chinese wolfberry, we optimized the culture medium(including hormone combination) and cul-ture conditions. The results showed that calluses were induced from all the six tested Chinese wolfberry materials, hut the induction rate of callus varied to-ward the materials with different genotypes. When the experimental materials were cuhured on medium appended with 2,4-D 1.0 mg/L and KT 1.0 mg/L un-der dark, the callus induction rate reached 20.0 % in this study, and this hormone combination should be the optimum for anther culture of Chinese wolf-berry. With MS appended with 6-BA 0.5 mg/L and NAA 0.1 mg/L as differentiation medium and that appended with NAA 0.1 mg/L, the plants could be yielded in 20 days. |
| |
| Keywords: | Lycium barbarum L Anther culture Callus tissue Regenerated plantlets |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
