非洲猪瘟病毒分子生物学检测方法研究进展

非洲猪瘟病毒感染猪后可引起猪高热、皮肤和内脏器官出血和高死亡率,死亡率可达100%。自2018年8月非洲猪瘟在中国首次报道以来,迅速席卷全国,给猪业造成严重的经济损失。文章综述6种非洲猪瘟病毒分子生物学检测方法,主要包括核酸探针、纳米PCR、荧光定量PCR、微滴数字PCR、环介导等温扩增技术、重组聚合酶扩增技术等。

Advances in Molecular Biological Detection Methods of African Swine Fever Virus

African swine fever virus can cause swine fever,skin and internal organ bleeding and high mortality after swine infection.The mortality rate can reach 100%.Since the first report of African swine fever in China in August 2018,it has quickly swept the country and given the swine industry Cause severe economic losses.This article reviews six molecular biological detection methods for African swine fever virus,including nucleic acid probes,nano-PCR,fluorescent quantitative PCR,droplet digital PCR,loop-mediated isothermal amplification technology,and recombinant polymerase amplification technology.