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水杉种子提取物抑菌机理研究
引用本文:杨俊杰,豆成林,李娟,王海燕.水杉种子提取物抑菌机理研究[J].信阳农业高等专科学校学报,2012,22(3):101-103,119.
作者姓名:杨俊杰  豆成林  李娟  王海燕
作者单位:信阳农业高等专科学校生物技术系,河南信阳,464000
基金项目:河南省科技攻关计划项目
摘    要:研究了水杉种子提取物的抑菌机理。方法:测定加入不同抑菌剂后的大肠杆菌液中还原糖含量、蛋白质含量以及电导率的变化。结果:水杉种子挥发油、总黄酮及提取物都具有一定的抑菌效果,抑菌效果随着浓度的增加显著提高,使大肠杆菌菌液的还原糖含量升高、蛋白质含量升高、电导率明显提高,其中以提取物的作用效果相对显著。结论:水杉种子提取物抑菌效果优于水杉种子挥发油、总黄酮,在天然产物抑菌剂开发方面前景广阔。

关 键 词:水杉种子  提取物  总黄酮  挥发油  抑菌机理

Study on the antibacterial mechanisms of the seed extracts of Metasequoia glyptostroboides
YANG Jun-jie,DOU Cheng-lin,LI Juan,WANG Hai-yan.Study on the antibacterial mechanisms of the seed extracts of Metasequoia glyptostroboides[J].Journal of Xinyang Agricultural College,2012,22(3):101-103,119.
Authors:YANG Jun-jie  DOU Cheng-lin  LI Juan  WANG Hai-yan
Institution:(Department of Biotechnology,Xinyang Agricultural College,Xinyang 464000,China)
Abstract:Object:To study the antibacterial mechanisms of the seed extracts of Metasequoia glyptostroboides.Methods:Test the changes of the reducing sugar content,the protein content,electric conductivity in the colon bacillus solution after being added different bacteriostatic agents.Result: All the essential oil,total flavones and compound extracts of the Metasequoia glyptostroboides seed have antibacterial effects,and the effects are better as the bacteriostatic agents’ consistency increased;and these three kinds of bacteriostatic agents can increase the reducing sugar content,the protein content,electric conductivity in the colon bacillus solution,especially the compound extracts of the Metasequoia glyptostroboides seed.Conclusion:The compound extracts of the Metasequoia glyptostroboides seed have better antibacterial effects than the essential oil,total flavones of it,and have good development prospects in the bacteriostatic action from natural products.
Keywords:Metasequoia glyptostroboides seed  extracts  total flavones  essential oil  antibacterial mechanisms
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