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| 应用改进的免疫荧光组织化学染色法对水稻根尖细胞微管结构的观察 | |
| 引用本文： | 计淑霞,盖国琛,戴绍军,刘炜.应用改进的免疫荧光组织化学染色法对水稻根尖细胞微管结构的观察[J].山东农业科学,2010(4):40-42,55. |
| 作者姓名： | 计淑霞 盖国琛 戴绍军 刘炜 |
| 作者单位： | 1. 哈尔滨师范大学生命科学与技术学院,黑龙江,哈尔滨,150025;山东省农业科学院高新技术研究中心/农业部黄淮海作物遗传改良与生物技术重点开放实验室,山东,济南,250100 2. 山东省农业科学院高新技术研究中心/农业部黄淮海作物遗传改良与生物技术重点开放实验室,山东,济南,250100 3. 哈尔滨师范大学生命科学与技术学院,黑龙江,哈尔滨,150025;东北林业大学生命科学学院,黑龙江,哈尔滨,150040 |
| 摘 要： | 植物细胞微管在细胞形态建成中具有重要作用。它体积小，且结构始终处于动态变化之中，因此观察到清晰的微管形态有一定的困难。本实验以水稻根尖为材料，介绍了一种改进的植物细胞微管免疫荧光组织化学染色方法，用此方法可以观察到清晰、完整的植物微管形态，此法对观察其它植物细胞微管结构也具有指导作用。 |
| 关 键 词： | 植物细胞 微管 水稻根尖 免疫荧光组织化学染色 |
An Improved Immunofluorescence Staining Method for Observing Microtubule Cytoskeleton in Root Tip of Rice |
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| JI Shu-xia,GAI Guo-chen,DAI Shao-jun,LIU Wei.An Improved Immunofluorescence Staining Method for Observing Microtubule Cytoskeleton in Root Tip of Rice[J].Shandong Agricultural Sciences,2010(4):40-42,55. | |
| Authors: | JI Shu-xia GAI Guo-chen DAI Shao-jun LIU Wei |
| Institution: | JI Shu-xia1,2,GAI Guo-chen2,DAI Shao-jun1,3,LIU Wei2(1.College of Life Science and Technology,Harbin Normal University,Harbin 150025,China,2.High-Tech Research Center,Shandong Academy of Agricultural Sciences/ Key Laboratory of Crop Genetic Improvement and Biotechnology,Huanghuaihai,Ministry of Agriculture,The People's Republic of China,Jinan 250100,3.College of Life Science,Northeast Forestry University,Harbin 150040,China) |
| Abstract: | The microtubule(MT) cytoskeleton plays an important role in plant cell morphogenesis.As the volume of MT is very small,and its morphology is always in dynamic changes,it is very difficult to observe a clear pattern of microtubule.In this paper,an improved immunofluorescence staining method was introduced using the root tip of rice as experimental materials,and the results showed that a clearly and fine structure of microtubule cytoskeleton could be observed.This method will play a guiding role in microtubul... |
| Keywords: | Plant cell Microtubule Root tip of rice Immunofluorescence staining method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |