| 反应温度和时间对分光光度法测定乳粉中左旋肉碱的影响 |
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| 引用本文： | 王文特,任雪梅,周传静,冯炜,田洪芸.反应温度和时间对分光光度法测定乳粉中左旋肉碱的影响[J].山东农业科学,2013(11):120-122. |
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| 作者姓名： | 王文特 任雪梅 周传静 冯炜 田洪芸 |
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| 作者单位： | 国家加工食品质量监督检验中心（山东）,山东济南250102 |
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| 摘 要： | 采用分光光度法测定乳粉中左旋肉碱的含量,从温度和时间对反应的影响进行分析研究,确立了最佳反应温度为24℃,最佳反应时间为加入5,5＇-二硫双（2-硝基苯甲酸）和乙酰辅酶A反应6 min后归零,再加入乙酰肉碱转移酶反应6 min。此条件下该方法的回收率为98.3%～99.7%。
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| 关 键 词： | 分光光度法 左旋肉碱 反应温度 反应时间 |
Effects of Reaction Temperature and Time on Spectrophotometric Determination of L-carnitine in Milk Powder |
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| Wang Wente;Ren Xuemei;Zhou Chuanjing;Feng Wei;Tian Hongyun.Effects of Reaction Temperature and Time on Spectrophotometric Determination of L-carnitine in Milk Powder[J].Shandong Agricultural Sciences,2013(11):120-122. |
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| Authors: | Wang Wente;Ren Xuemei;Zhou Chuanjing;Feng Wei;Tian Hongyun |
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| Institution: | Wang Wente;Ren Xuemei;Zhou Chuanjing;Feng Wei;Tian Hongyun;National Processed Food Quality Supervision and Inspection Center (Shandong); |
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| Abstract: | The spectrophotometric method was used to detect L-carnitine content in milk powder,and the effects of reaction temperature and time were analyzed. The experiment results showed that the optimal reaction temperature was 24℃; the optimal reaction time was 6 minutes after adding 5,5＇-two sulfur duoble（ 2-nitrobenzoic acid） and acetyl coenzyme A,then adjusted to zero,and another 6 minutes after adding acetyl carnitine transferase. Under these conditions,the recovery was 98. 3% ～ 99. 7%. |
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| Keywords: | Spectrophotometric method L-carnitine Reaction temperature Reaction time |
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