陶瓷膜超滤纯化香菇多糖及其相对分子质量的测定

采用截留分子量为104u的陶瓷超滤膜纯化香菇多糖溶液,考察运行时间、跨膜压差（TMP）、稀释倍数和操作温度对膜通量的影响,分析各条件下稳定通量的变化规律。结果表明：实验设备运行1 h后膜管通量开始稳定,当跨膜压差为0.08 MPa、料液稀释倍数为3倍、操作温度50℃时,稳定通量维持在75 L/（h·m2）。通过超滤纯化制得的香菇多糖的纯度为89.7%,高效凝胶渗透色谱法测得平均相对分子量为2.27×106u,并对所得香菇多糖的光谱学性质进行了研究。

Purification of Mushroom Polysaccharide by Ceramic Membrane and Determination of Its Molecular Weight

The ceramic membrane with molecular weight cut off 104u was used to purify mushroom polysaccharide,and the effects of reaction time,trans-membrane pressure（ TMP）,dilution times,and operation temperature on permeation flux and its change law were analyzed. The results showed that the permeation flux tended to be stable after equipment running for 1 hour. A stable permeation flux of 75 L/（ h·m2） was obtained when pertransmembrane pressure was 0. 08 MPa,dilution times was 3 and operation temperature was50℃. The purity of mushroom polysaccharide obtained through purification was 89. 7%. Its average relative molecular weight was 2. 27 × 106u measured by HPGFC. And its spectral properties were also investigated.