| 烟粉虱刺吸诱导转Bt＋CpTI基因棉苯丙氨酸解氨酶和氧保护酶系活性变化 |
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| 引用本文： | 姜涛,褚栋,姜德锋,陶云荔,刘国霞,范仲学.烟粉虱刺吸诱导转Bt＋CpTI基因棉苯丙氨酸解氨酶和氧保护酶系活性变化[J].山东农业科学,2009(10):48-53. |
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| 作者姓名： | 姜涛 褚栋 姜德锋 陶云荔 刘国霞 范仲学 |
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| 作者单位： | 1. 山东省农业科学院高新技术研究中心/山东省作物与畜禽品种改良生物技术重点实验室,山东济南250100;农业部黄淮海作物遗传改良与生物技术重点开放实验室,山东济南250100
2. 青岛农业大学农学与植物保护学院,山东青岛,266109 |
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| 基金项目： | 山东省农业科学院高技术创新基金，国家科技支撑计划，山东省优秀中青年科学家科研奖励基金 |
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| 摘 要： | 本研究测定并比较了不同时期转双价基因棉SGK321和其亲本常规棉SY321叶片中苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的总活性。结果发现，烟粉虱取食诱导使棉叶中PAL、POD的活性上升，SOD的活性则是先下降后升高，CAT的活性基本呈下降趋势。SGK321处理植株叶片中PAL活性在不同时期均高于SY321，而POD活性在不同时期均高于SY321；SGK321处理植株上部叶片SOD活性在处理30d前明显高于SY321，40d时低于SY321；SGK321处理植株叶片中CAT活性多数时期低于SY321。最后，探讨了转双价基因棉花与其亲本常规棉对烟粉虱防御反应的差异。
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| 关 键 词： | Bt+CpTI基因棉 烟粉虱 苯丙氨酸解氨酶 保护酶系统 |
Activity Dynamics of Phenylalanine Ammonia-lyase and Protective Enzymes in Transgenic Bt+CpTI Cotton Induced by Bemisia tabaci Feeding |
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| JIANG Tao,CHU Dong,JIANG De-Feng,TAO Yun-Li,LIU Guo-Xia,FAN Zhong-Xue.Activity Dynamics of Phenylalanine Ammonia-lyase and Protective Enzymes in Transgenic Bt+CpTI Cotton Induced by Bemisia tabaci Feeding[J].Shandong Agricultural Sciences,2009(10):48-53. |
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| Authors: | JIANG Tao CHU Dong JIANG De-Feng TAO Yun-Li LIU Guo-Xia FAN Zhong-Xue |
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| Institution: | JIANG Tao, CHU Dong, JIANG De - Feng, TAO Yun - Li, LIU Guo - Xia, FAN Zhong - Xue ( 1. High - tech Research Center, Shandong Academy of Agricultural Sciences/Key Laboratory for Genetic Imprrovement of Crop Animal and Poultry of Shandong Province, Jinan 250100, China; 2. Key laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, the People＇s Republic of China, Jinan 250100, China; 3. College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, China) |
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| Abstract: | The activities of phenylalanine ammonia-lyase(PAL) and protective enzymes(POD,SOD and CAT) in leaves of SGK321 and SY321 damaged by B.tabaci were determined in different time.The results showed that the activities of PAL and POD was rising,SOD activity was dropping-rising and CAT was dropping.The PAL activity in SGK321 leaves was lower than that in SY321 during different phases while the POD activity of SGK321 was higher.The activity of SOD in SGK321 was obviousty higher than that in SY321 in the 30 days after treatment,while lower after 40 days.The activity of CAT in SGK321 was lower than that in SY321.Finally,the differences of the defense response between the transgenic Bt+CpTI cotton SGK321 and its parent SY321 were discussed. |
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| Keywords: | Transgenic Bt+CpTI cotton Bemisia tabaci Gennadius Phenylalanine ammonia-lyase Protective enzymes |
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