| 小麦矮秆新基因的SSR标记 |
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| 引用本文： | 石涛,王洪刚,何方,邓世民,高居荣.小麦矮秆新基因的SSR标记[J].山东农业科学,2008(8). |
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| 作者姓名： | 石涛 王洪刚 何方 邓世民 高居荣 |
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| 作者单位： | 1. 山东农业大学农学院/国家小麦改良中心泰安分中心,山东,泰安,271018
2. 山东农业大学农学院/国家小麦改良中心泰安分中心,山东,泰安,271018;山东农业大学/作物生物学国家重点实验室,山东,泰安,271018 |
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| 基金项目： | 国家"十一五"科技支撑计划 |
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| 摘 要： | 以小麦矮秆种质系山农31504-1作父本与中国春杂交,获得F2分离群体。利用定位于2A染色体上的179对SSR、EST-SSR引物,采用BSA法,对亲本及197株F2分离群体单株进行SSR分析,筛选出2个与矮秆基因紧密连锁的SSR标记(Xwm c522、Xwm c198),其遗传距离分别为5.4 cM、3.2 cM,可用于分子标记辅助选择。
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| 关 键 词： | 小麦 矮秆基因 SSR BSA |
Identification of SSR Markers Linked to New Wheat Dwarf Gene |
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| SHI Tao,WANG Hong-gang,HE Fang,DENG Shi-min,GAO Ju-rong.Identification of SSR Markers Linked to New Wheat Dwarf Gene[J].Shandong Agricultural Sciences,2008(8). |
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| Authors: | SHI Tao WANG Hong-gang HE Fang DENG Shi-min GAO Ju-rong |
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| Institution: | SHI Tao1,WANG Hong-gang1,2,HE Fang1,DENG Shi-min1,GAO Ju-rong1 |
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| Abstract: | In this study,the cross between dwarf germplasm line Shannong 31504-1 and Chinese Spring was carried out for constructing F2 segregation population.By Bulked segregating analysis(BSA) and SSR analysis with 179 pairs of SSR and EST-SSR primer located on 2A chromesome on 197 individuals of F2 segregation population and their parents,two SSR markers(Xwmc522 and Xwmc198) that linked to the dwarf gene in Shannong 31504-1 were screened.The dwarf gene was shown to be closely linked to the marker Xwmc522 and Xwmc198 at a genetic distance of 5.4 cM and 3.2 cM respectively.They could be used in marker-assisted selection directly. |
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| Keywords: | Wheat Dwarf gene SSR BSA |
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