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利用花粉管通道技术创造棉花变异种质及其SSR标记分析
引用本文:王芙蓉,张传云,刘国栋,刘任重,刘勤红,张军.利用花粉管通道技术创造棉花变异种质及其SSR标记分析[J].山东农业科学,2006(1):10-12.
作者姓名:王芙蓉  张传云  刘国栋  刘任重  刘勤红  张军
作者单位:山东棉花研究中心,山东,济南,250100
基金项目:山东省优秀中青年科学家科研奖励基金;山东省科技厅资助项目
摘    要:将海岛棉品种海7124的基因组总DNA通过花粉管通道导入到陆地棉品种石远321中,获得了纤维品质明显改善的优良新种质系,而且在后代材料中还获得了一个形态性状发生明显变异的突变体。利用SSR标记对DNA供体、受体及突变体二代材料进行了多态性分析,结果表明,海岛棉DNA片段已经整合到受体基因组中,这为花粉管通道技术提供了间接的分子生物学证据,同时对外源DNA片段的整合机制进行了探讨。

关 键 词:棉花  花粉管通道技术  变异种质  SSR标记
文章编号:1001-4942(2006)01-0010-03
收稿时间:2005-08-23
修稿时间:2005年8月23日

Variant germplasm obtained by pollen tube pathway technique and SSR analysis
WANG Fu-rong,ZHANG Chuan-yun,LIU Guo-dong,LIU Ren-zhong,LIU Qin-hong,ZHANG Jun.Variant germplasm obtained by pollen tube pathway technique and SSR analysis[J].Shandong Agricultural Sciences,2006(1):10-12.
Authors:WANG Fu-rong  ZHANG Chuan-yun  LIU Guo-dong  LIU Ren-zhong  LIU Qin-hong  ZHANG Jun
Institution:Cotton Reseach Center,Shandong Academy of Agricultural Sciences, Jinan 250100,China
Abstract:The genomic DNA of island cotton(Gossypium barbadense L.cv.Hai7124) was introduced into the upland cotton(G.hirsutum L.cv.Shiyuan321) by pollen tube pathway technique.New germplasm of high-quality fiber and a mutant with obvious change of morphology were obtained.The result of SSR(simple sequence repeats) analysis indicated that exogenous DNA fragments were integrated into the genome of upland cotton Shiyuan321.It is supposed that some of the DNA fragments were integrated by homologous recombination mechanism.
Keywords:Cotton  DNA introduction by pollen tube pathway  Variant germplasm  SSR marker
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