| 玉米单倍体高效加倍技术规范 |
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| 引用本文： | 李杰文,刘长虹,刘治先,杨菲,李文才,孙琦,于艳丽,丁照华.玉米单倍体高效加倍技术规范[J].山东农业科学,2013(10):121-124,130. |
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| 作者姓名： | 李杰文 刘长虹 刘治先 杨菲 李文才 孙琦 于艳丽 丁照华 |
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| 作者单位： | 山东省农业科学院玉米研究所; |
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| 基金项目： | 国家高技术研究发展计划(863计划)“黄淮海夏玉米区强优势玉米杂交种的创制与应用”(2011AA10A103-4);济南市高校院所自主创新项目“玉米单倍体育种技术在抗粗缩病品种选育上的应用”(201102067) |
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| 摘 要： | 根据多年来玉米单倍体育种研究的理论和实践，结合国内外单倍体育种的成功经验，组装集成了“玉米单倍体高效加倍技术规范”（仅供参照试用）。本规范是玉米单倍体人工（化学）加倍的基本操作程序，适用于利用杂交诱导单倍体快速选育玉米自交系技术，主要包括单倍体种植、单倍体植株确认、高效加倍技术、单倍体植株的授粉与收获、获得DH系等5个技术环节。按此技术规程操作，可显著提高单倍体的加倍率和育种成效。
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| 关 键 词： | 玉米单倍体 标记性状 DH系 化学加倍 注意事项 |
Technical Specifications for Efficient Doubling of Maize Haploid |
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| Li Jiewen,Liu Changhong,Liu Zhixian,Yang Fei,Li Wencai,Sun Qi,Yu Yanli,Ding Zhaohua.Technical Specifications for Efficient Doubling of Maize Haploid[J].Shandong Agricultural Sciences,2013(10):121-124,130. |
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| Authors: | Li Jiewen Liu Changhong Liu Zhixian Yang Fei Li Wencai Sun Qi Yu Yanli Ding Zhaohua |
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| Institution: | (Maize Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China) |
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| Abstract: | Based on years of research on theory and practice of maize haploid breeding, the technical specifications for efficient doubling of maize haploid （ only for reference） had been assembled combined with the successful experience at home and abroad. The specifications, as the basal operation process of artificial doubling of maize haploid, applied to the technique of inducing haploid by crossing for rapidly breeding maize inbred lines ; it mainly included 5 technical links as cultivation of haploids, confirmation of haploid plants, ef- ficient doubling techniques, pollination and harvest of haploid plants and obtaining DH lines. Using the tech- nical specifications could significantly enhance the doubling rate and breeding effect of haploid. |
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| Keywords: | Maize haploid Marker character Doubled haploid line Chemical doubling Matters needing attention |
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