| 快速蛋白液相色谱分离小麦高分子量谷蛋白亚基方法的研究 |
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| 引用本文： | 李玉冰,裴玉贺,王海娟,郭新梅,张恩盈,宋希云.快速蛋白液相色谱分离小麦高分子量谷蛋白亚基方法的研究[J].山东农业科学,2009(5):98-101. |
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| 作者姓名： | 李玉冰 裴玉贺 王海娟 郭新梅 张恩盈 宋希云 |
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| 作者单位： | 青岛农业大学生命科学学院,山东,青岛,266109 |
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| 基金项目： | 国家科技支撑计划粮食丰产科技工程项目 |
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| 摘 要： | 高分子量谷蛋白亚基作为小麦贮藏蛋白的重要组成部分，其组成和含量对小麦面粉的烘烤品质和加工品质有重要影响。本研究以普通小麦品种小偃22号为材料，采用快速蛋白液相色谱（Fast Protein Liquid Chromatograth，FPLC）凝胶过滤来分离高分子量谷蛋白亚基，并从样品浓度、洗脱液和流速3个方面进行了研究，发现将原样品稀释8倍、洗脱液为尿素、流速为0．3ml／min时洗脱效果最好。
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| 关 键 词： | 小麦 高分子量谷蛋白亚基 凝胶过滤 快速蛋白液相色谱 |
Elementary Exploration on Method for Separating Wheat HMW-GS by FPLC |
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| LI Yu-bing,PEI Yu-he,WANG Hai-juan,GUO Xin-mei,ZHANG En-ying,SONG Xi-yun.Elementary Exploration on Method for Separating Wheat HMW-GS by FPLC[J].Shandong Agricultural Sciences,2009(5):98-101. |
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| Authors: | LI Yu-bing PEI Yu-he WANG Hai-juan GUO Xin-mei ZHANG En-ying SONG Xi-yun |
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| Institution: | LI Yu -bing, PEI Yu -he, WANG Hai -juan, GUO Xin -mei, ZHANG En -ying, SONG Xi -yun( College of Life Science,Qingdao Agricultural University, Qingdao 266109, China) |
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| Abstract: | As major components of wheat storage proteins,the compositions and content of high molecular weight glutenin subunit(HMW-GS) play an important role in wheat baking and processing quality.HMW-GS was separated and analyzed by gel filter with liquid chromatography methods from the common wheat Xiaoyan22.Different concentration,elution and flow rate were studied in this experiment.It was found that the concentration diluted eight times from original concentration was the best.Urea was the best elution and 0.3 ml/min was the best flow rate. |
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| Keywords: | Wheat HMW-GS Gel filter FPLC |
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