| 谷子种质资源遗传多样性研究 |
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| 引用本文： | 孙加梅,王雪梅,王东建,杨延兵,李华,段丽丽,王晓宇,张晗.谷子种质资源遗传多样性研究[J].山东农业科学,2013,45(3):33-37,60. |
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| 作者姓名： | 孙加梅 王雪梅 王东建 杨延兵 李华 段丽丽 王晓宇 张晗 |
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| 作者单位： | 1. 山东省农业科学院作物研究所,山东济南,250100
2. 山西省农业科学院谷子研究所,山西长治,046011 |
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| 基金项目： | 山东省自然科学基金项目"谷子基因组SSR序列的分离、鉴定和引物开发" |
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| 摘 要： | 利用谷子基因组序列开发出SSR引物205对,其中171对可以稳定扩增出目的条带,149对有多态性。利用30个多态性SSR标记对41份谷子种质资源进行遗传多样性分析,共检测出291个等位变异,平均每个位点9.7个。谷子地方品种基因多样性指数平均为0.7907,育成品种基因多样性指数平均为0.7571。对41个谷子品种进行聚类,在遗传相似系数为0.164时聚为6组,与生态型基本一致。
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| 关 键 词： | 谷子 SSR标记 遗传多样性 聚类分析 |
Genetic Diversity Analysis of Foxtail Millet |
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| Sun JiaMei,Wang XueMei,Wang DongJian,Yang YanBing,Li Hua,Duan LiLi,Wang XiaoYu,Zhang Han.Genetic Diversity Analysis of Foxtail Millet[J].Shandong Agricultural Sciences,2013,45(3):33-37,60. |
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| Authors: | Sun JiaMei Wang XueMei Wang DongJian Yang YanBing Li Hua Duan LiLi Wang XiaoYu Zhang Han |
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| Institution: | 1**(1.Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China; 2.Millet Research Institute,Shanxi Academy of Agricultural Sciences,Changzhi 046011,China) |
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| Abstract: | In this study,205 SSR primer pairs were designed from the genome sequence of foxtail millet.In which,171 pairs could successfully amplify aimed fragment,and 149 pairs showed polymorphism.41 foxtail millet germplasms were selected for genetic diversity analysis using 30 SSR markers.A total of 291 alleles were detected out with an average of 9.7 per locus.The average genetic diversity index was 0.7907 for landraces and 0.7571 for improved varieties.Cluster analysis showed that the 41 foxtail millet varieties were divided into six groups at the similarity coefficient of 0.164,which were consistent with the ecotypes of these varieties. |
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| Keywords: | Foxtail millet SSR markers Genetic diversity Cluster analysis |
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