利用SSR标记进行小麦品种鉴定和新品种保护研究

利用变性聚丙烯酰胺电泳结合银染技术,检测了8个SSR位点在71份中国育成小麦品种(系)中的多态性,确定了各SSR位点上等位基因的大小。在71份小麦品种(系)中,共检测到等位基因75个,每对引物检测到的等位基因个数最少为6个(gwm186),最多为15个(gwm174),平均9.4个。PIC值最高为0.8739(gwm174),最低为0.6552(gwm186),平均为0.7968。利用少数几对高PIC值的SSR引物,能够将全部71份品种(系)区分开来,供试材料间至少有一对等位基因存在差异。对SSR标记应用于小麦品种鉴定和品种保护的可行性进行了讨论。

Application of SSR Markers to Identification and Protection of Wheat Variety

In this study,the diversity and size of allele at 8 SSR loci among 71 Chinese winter wheat cultivars(lines) were determined using denaturing PAGE and silver staining.75 alleles in total were detected,ranging from 6(gwm186) to 15(gwm174) per primer with the average of 9.4.The PIC values of the SSR loci varied from the lowest of 0.6552(gwm186) to the highest of 0.8739(gwm174) and averaged 0.7968.Genotypes of each variety consisting of allele sizes at 8 SSR loci were established.All the 71 cultivars(lines) could be uniquely identified and distinguished from each other using the 8 SSR markers,and there were at least one allele different from each other.SSR markers are especially suitable for identifying wheat cultivars(lines) developed through conventional hybridization breeding methods.