| 黑树莓茎尖组织培养研究 |
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| 引用本文： | 孟静静,陈传英,李慧,郭峰,徐平丽,张悦丽,李新国.黑树莓茎尖组织培养研究[J].山东农业科学,2010(8):8-10. |
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| 作者姓名： | 孟静静 陈传英 李慧 郭峰 徐平丽 张悦丽 李新国 |
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| 作者单位： | 山东省农业科学院高新技术研究中心,山东,济南,250100 |
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| 基金项目： | 山东省自然科学基金项目，博士后创新项目专项资金，山东省农业科学院青年科研基金 |
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| 摘 要： | 取春季黑树莓的嫩枝条,以茎尖为外植体,MS为基本培养基,添加植物激素6-BA、NAA、GA3筛选培养基,进行茎尖组织培养研究。试验结果表明,诱导茎尖萌发的最佳培养基为MS＋0.5 mg/L 6-BA＋0.05 mg/L NAA,萌发率高达90%;继代培养最佳培养基为MS＋1.0 mg/L 6-BA＋0.02 mg/L NAA＋6.0 mg/L GA3,繁殖系数达6.0～7.0;生根最佳培养基为MS＋0.5 mg/L6-BA＋0.1 mg/L NAA,生根率为96%。
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| 关 键 词： | 黑树莓 茎尖 组织培养 |
Study on Shoot Tip Culture of Black Raspberry |
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| MENG Jing-jing,CHEN Chuan-ying,LI Hui,GUO Feng,XU Ping-li,ZHANG Yue-li,LI Xin-guo.Study on Shoot Tip Culture of Black Raspberry[J].Shandong Agricultural Sciences,2010(8):8-10. |
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| Authors: | MENG Jing-jing CHEN Chuan-ying LI Hui GUO Feng XU Ping-li ZHANG Yue-li LI Xin-guo |
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| Institution: | MENG Jing-jing,CHEN Chuan-ying,LI Hui,GUO Feng,XU Ping-li,ZHANG Yue-li,LI Xin-guo(High-Tech Research Center,Shandong Academy of Agricultural Sciences,Jinan 250100,China) |
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| Abstract: | The shoot tips of black raspberry in spring were used as the explants for tissue culture in the MS basic medium supplemented with 6-BA,NAA and GA3.The best medium for inducing germination of shoot tip was MS+0.5 mg/L 6-BA+0.05 mg/L NAA,in which the germination rate was about 90%.The best medium for subculture was MS+1.0 mg/L 6-BA+0.02 mg/L NAA+6.0 mg/L GA3,in which the propagation coefficient was 6.0～7.0.Additionally,the best rooting medium was MS+0.5 mg/L 6-BA+0.1mg/L NAA,in which the rooting rate was about 96%. |
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| Keywords: | Black raspberry Shoot tip Tissue culture |
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