| 药用植物刺五加组织培养及快速繁殖的研究 |
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| 引用本文： | 韩宏义,郑静,白鹏.药用植物刺五加组织培养及快速繁殖的研究[J].山东农业科学,2008(2):18-20. |
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| 作者姓名： | 韩宏义 郑静 白鹏 |
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| 作者单位： | 丹东市农业科学院生物技术研究中心,辽宁,丹东,118109 |
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| 摘 要： | 选用刺五加的茎尖为外植体材料,采用正交试验方法研究了激素对刺五加愈伤组织分化、继代培养、不定芽发生及生根的影响。试验结果表明,刺五加的最佳初分化培养基为MS+6-BA2.0 mg/L+KT0.8mg/L+NAA0.10~0.15 mg/L,继代芽分化培养基MS+6-BA0.5 mg/L+IAA1 mg/L+IBA0.8 mg/L,生根培养基为1/2MS+IBA0.4 mg/L
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| 关 键 词： | 刺五加 组织培养 正交试验 激素 继代培养 |
| 文章编号： | 1001-4942(2008)02-0018-03 |
| 修稿时间： | 2007年11月9日 |
Tissue Culture and Rapid Breeding of Acanthopanax senticosus |
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| HAN Hong-yi,ZHENG Jing,BAI Peng.Tissue Culture and Rapid Breeding of Acanthopanax senticosus[J].Shandong Agricultural Sciences,2008(2):18-20. |
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| Authors: | HAN Hong-yi ZHENG Jing BAI Peng |
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| Abstract: | Acanthopanax senticosus buds were used as research materials.It was determined by the orthogonal test that callus differentiation,subculture,shoot and root induction of the Acanthopanax senticosus were influenced by different hormone combinations of culture media.The results showed MS+6-BA 2.0 mg/L+KT 0.8 mg/L+NAA 0.10～0.15 mg/L was the most favorable medium for differentiation of Acanthopanax senticosus tissue culture,MS+6-BA 0.5 mg/L+ IAA 1 mg/L+IBA 0.8 mg/L was the one for subculture of regeneration bud,and 1/2MS+IBA 0.4 mg/L was the one for root induction. |
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| Keywords: | Acanthopanax senticosus Tissue culture Orthogonal test Hormone Subculture |
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