| 苹果渣固态发酵产复合酶培养基优化的研究 |
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| 引用本文： | 姜心,陈伟,周波.苹果渣固态发酵产复合酶培养基优化的研究[J].山东农业科学,2010(1):80-85. |
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| 作者姓名： | 姜心 陈伟 周波 |
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| 作者单位： | 1. 山东农业大学食品科学与工程学院,山东,泰安,271018
2. 山东农业大学生命科学学院,山东,泰安,271018 |
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| 基金项目： | 国家科技基础条件平台“微生物菌种资源整理整合项目(2005DKA21201-13)”;;国家科技支撑计划“黄淮平原区农田秸秆资源综合利用技术集成研究与示范(2007BAD89B09-16)”资助 |
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| 摘 要： | 以黑曲霉C-2为菌种，对苹果渣进行固态发酵，以纤维素酶、木聚糖酶、果胶酶的活性为测定指标，单因素试验确定水平，采用L9（3^4）正交试验对产复合酶体系的固态培养基质进行优化，同时运用极差分析、方差分析以及不同因素水平间差异显著性检验对结果进行分析，以得到较合理的培养基。优化后培养基组成为果渣：麸皮：4：1，2．0％硫酸铵，0．1％磷酸氢二钾，2．5％尿素。优化后三种酶活力比基础培养基分别提高了64．19％、17．26％、20．89％。
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| 关 键 词： | 苹果渣 固态发酵 复合酶 培养基 优化 |
Optimization of Solid-state Fermentation Medium for Production of Complex Enzymes with Apple Pomace |
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| JIANG Xin,CHEN Wei,ZHOU Bo.Optimization of Solid-state Fermentation Medium for Production of Complex Enzymes with Apple Pomace[J].Shandong Agricultural Sciences,2010(1):80-85. |
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| Authors: | JIANG Xin CHEN Wei ZHOU Bo |
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| Institution: | 1.College of Food Science and Engineering;Shandong Agricultural University;Taian 271018;China;2.College of Life Science;China |
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| Abstract: | With the activity of cellulase,xylanase and pectinase as the determination indicators,the optimization of medium composition was investigated in solid-state fermentation of apple pomace by Aspergillus niger C-2.The culture medium was optimized using L9(34) orthogonal design,range analysis,variance analysis and test of significance between the levels of different factors,so as to get a more reasonable result.As the result,the optimized medium composition was apple pomace∶bran = 4∶1,ammonium sulfate of 2.0%,potassium hydrogen phosphate of 0.1%,urea of 2.5%.Compared with the basic medium,the activity of three enzyme was increased by 64.19%,17.26% and 20.89%,respectively. |
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| Keywords: | Apple pomace Solid-state fermentation Complex enzyme Medium Optimization |
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