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萝卜雄性不育系与保持系的生理生化指标分析
引用本文:韩晓雨,王淑芬,魏佑营,刘贤娴,徐文玲,付卫民.萝卜雄性不育系与保持系的生理生化指标分析[J].山东农业科学,2012,44(12):36-38.
作者姓名:韩晓雨  王淑芬  魏佑营  刘贤娴  徐文玲  付卫民
作者单位:1. 山东农业大学园艺科学与工程学院,山东泰安,271018
2. 山东省农业科学院蔬菜研究所/国家蔬菜改良中心山东分中心,山东济南,250100
基金项目:山东省现代农业产业技术体系项目,山东省农业重大应用技术创新项目共同资助
摘    要:以萝卜雄性不育系及其保持系为试材,测定了不同发育时期叶片、花和花蕾中脯氨酸、可溶性蛋白、可溶性糖、丙二醛、超氧阴离子以及SOD、POD、CAT的活性。结果表明,盛花期花蕾和花朵中,不育系脯氨酸、可溶性蛋白、可溶性糖的含量均低于保持系,丙二醛和超氧阴离子含量高于保持系,SOD、POD和CAT酶活性变化与萝卜的雄性不育相关。

关 键 词:萝卜  雄性不育  生理生化指标  超氧阴离子  酶活性

Physiological and Biochemical characterization of Radish Male-Sterile Line and Its Maintainer Line
Han XiaoYu , Wang ShuFen , Wei YouYing , Liu XianXian , Xu WenLing , Fu WeiMin.Physiological and Biochemical characterization of Radish Male-Sterile Line and Its Maintainer Line[J].Shandong Agricultural Sciences,2012,44(12):36-38.
Authors:Han XiaoYu  Wang ShuFen  Wei YouYing  Liu XianXian  Xu WenLing  Fu WeiMin
Institution:1(1.College of Horticulture Science and Engineering,Shandong Agricultural University,Taian 271018,China; 2.Vegetable Research Institute,Shandong Academy of Agricultural Sciences/Shandong Branch of State Vegetable Improvement Center,Jinan 250100,China)
Abstract:With the male sterile line and its maintainer line as test materials,the content of free proline,soluble protein,soluble sugar,MDA and superoxide anion and the activity of SOD,POD and CAT were determined in leaves,flowers and buds at different developing stages.The results showed that in buds and flowers at full-bloom stage,the content of proline,soluble protein and soluble sugar in sterile line were lower than those in maintainer line,while the contents of MDA and superoxide anion were higher than those in maintainer line.The changes in activity of SOD,POD and CAT were correlated with the radish male sterility.
Keywords:Raphanus sativus L    Male sterility  Physiological and biochemical indicators  Superoxide anion  Enzyme activity
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