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| 榅桲离体培养繁殖的研究 | |
| 引用本文： | 魏国芹,戴洪义,孙玉刚,梁美霞,安淼.榅桲离体培养繁殖的研究[J].山东农业科学,2010(1):5-9. |
| 作者姓名： | 魏国芹 戴洪义 孙玉刚 梁美霞 安淼 |
| 作者单位： | 1. 青岛农业大学园林园艺学院,山东,青岛,266109;山东省果树研究所,山东,泰安,271000 2. 青岛农业大学园林园艺学院,山东,青岛,266109 3. 山东省果树研究所,山东,泰安,271000 |
| 摘 要： | 利用榅桲茎尖进行继代培养，建立了榅桲的离体再生体系。结果表明，适合离体叶片愈伤组织诱导的培养基为1／2MS＋KT0．5mg／L＋2，4-D1．0mg／L＋蔗糖30．0g／L＋琼脂6．5g／L；适合愈伤组织分化不定芽的培养基为MS＋TDZ3．0mg,／L＋NAA0．3mg／L＋蔗糖30．0g／L＋琼脂6．5g／L；适合叶片再生不定芽的培养基为MS＋TDZ2．0mg／L＋NAA0．1mg／L＋蔗糖30．0g／L＋琼脂6．5g／L；适合榅桲茎段生根的培养基为1／2MS＋IAA0．3mg／L＋蔗糖30．0g／L＋琼脂6．5g／L。 |
| 关 键 词： | 榅桲 愈伤诱导 植株再生 生根诱导 |
Research on Propagation in Vitro of Quince |
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| WEI Guo-qin,DAI Hong-yi,SUN Yu-gang,LIANG Mei-xia,AN Miao.Research on Propagation in Vitro of Quince[J].Shandong Agricultural Sciences,2010(1):5-9. | |
| Authors: | WEI Guo-qin DAI Hong-yi SUN Yu-gang LIANG Mei-xia AN Miao |
| Institution: | 1.College of Horticulture;Qingdao Agricultural University;Qingdao 266109;China;2.Shandong Institute of Pomology;Taian 271000;China |
| Abstract: | The regeneration system in vitro of quince(Cydonia oblonga Mill.) was established.The results indicated that the optimal medium for calli induction via leaves was 1/2MS+KT 0.5 mg/L+ 2,4-D 1.0 mg/L+sucrose 30.0 g/L+agar 6.5 g/L.The optimal medium for the differentiation of calli was 1/2MS+TDZ 3.0 mg/L+NAA 0.3 mg/L + sucrose 30.0 g/L+agar 6.5 g/L.The optimal medium for plant regeneration via leaves was MS+TDZ 2.0 mg/L+NAA 0.1 mg/L+sucrose 30.0 g/L+agar 6.5 g/L.The optimal medium for root induction was 1/2MS+I... |
| Keywords: | Quince Callus induction Plant regeneration Root induction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |