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牡丹根腐病拮抗菌的筛选与鉴定
引用本文:王雪山,王善林,杜秉海,姚良同,丁廷芹.牡丹根腐病拮抗菌的筛选与鉴定[J].山东农业科学,2012,44(7):90-94.
作者姓名:王雪山  王善林  杜秉海  姚良同  丁廷芹
作者单位:1. 山东农业大学生命科学学院微生物系,山东泰安,271018
2. 山东农业大学生命科学学院微生物系,山东泰安271018;山东省农业微生物重点实验室,山东泰安271018
摘    要:从菏泽曹州牡丹园采集根际土壤样品6个,从中分离出300个分离物,以牡丹根腐病病原菌(Fusarium solani)为靶标菌,通过平板对峙法筛选出27个具有拮抗性能的菌株,其中MD1、MD4、MD7、MD8、MD64、MD68、MD70具有较强拮抗能力,且拮抗性能稳定。经过形态观察、生理生化特性分析及16S rDNA序列分析,将MD1鉴定为Bacillus amyloliquefaciens,MD4鉴定为Bacillus malacitensis,MD7鉴定为Bacillus subtilis,MD8鉴定为Pseudomonas chlororaphis;MD64鉴定为Streptomyces albireticuli;MD68鉴定为Bacillus tequilensis;MD70鉴定为Bacillus methylotrophicus。它们的16S rDNA序列在GenBank中注册号分别为JN205338、JN205339、JN205340、JN205341、JN205342、JN205343、JN205344。

关 键 词:牡丹根际  茄病镰孢霉菌  拮抗菌  筛选  鉴定

Screening and Identification of Antagonistic Strains against Fusarium solani Causing Tree Peony Root Rot
WANG Xue-shan , WANG Shan-lin , DU Bing-hai , YAO Liang-tong , DING Yan-qin.Screening and Identification of Antagonistic Strains against Fusarium solani Causing Tree Peony Root Rot[J].Shandong Agricultural Sciences,2012,44(7):90-94.
Authors:WANG Xue-shan  WANG Shan-lin  DU Bing-hai  YAO Liang-tong  DING Yan-qin
Institution:1 (1.Department of Microbiology,College of Life Science,Shandong Agricultural University,Taian 271018,China; 2.Shandong Key Laboratory for Agricultural Microbiology,Taian 271018,China)
Abstract:Six soil samples were collected from tree peony rhizosphere in Heze,and 300 strains belong to bacteria or actinomyces were isolated.Based on the antagonistic analysis using Fusarium solani as target strain,27 isolates were found with the antagonistic ability,and in which,MD1,MD4,MD7,MD8,MD64,MD68 and MD70 had the strongest antagonistic properties.Based on their morphological,physiological and phenotypic characteristics and 16S rDNA sequence,they were identified as Bacillus amyloliquefaciens,Bacillus malacitensis,Bacillus subtilis,Pseudomonas chlororaphis,Streptomyces albiroticuli,Bacillus tequilensis and Bacillus methylotrophicus respectively.Their 16S rDNA sequences had been submitted to GenBank and their accession numbers were JN205338,JN205339,JN205340,JN205341,JN205342,JN205343 and JN205344 respectively.
Keywords:Tree peony rhizosphere  Fusarium solani  Antagonistic bacteria  Screening  Identification
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