雪松松针总黄酮醇提工艺优化

利用乙醇浸提法提取雪松松针中的黄酮类化合物,以芦丁为对照品,采用NaAc-AlCl3比色法考察总黄酮提取率,通过单因素试验和正交试验对提取工艺进行优化。结果表明,该方法的最佳提取工艺为:以1 g松针为原料,按照1∶45的料液比加入50%乙醇作为提取剂,于75℃恒温回流提取2 h。最佳工艺条件下雪松松针的总黄酮提取率为3.64%,RSD为4.04%。该方法稳定可靠,工艺合理可行。

Optimization of Alcohol Extraction Technology of Total Flavonoids from Cedar Pine Needles

The total flavonoids in cedar pine needles were extracted with ethyl alcohol. Using rutin as reference substance, the total extraction rate was studied by NaAc - A1C13 colorimetry. The extraction technol- ogy was optimized through a series of single factor experiments and orthogonal experiment. The results showed that the optimum extraction process was as follows：l g pine needle sample as raw material, 50% ethanol solution as extractant added at the ratio of material to solvent as 1 ： 45, 2 hours＇ reflux extraction at 75 %. Under the optimum conditions, the extraction rate of total flavonoids from cedar pine needles was 3.64% with the RSD as 4.04%. This method was reliable, reasonable and feasible.