| 基于量子点的赭曲霉毒素AsFLISA检测技术研究 |
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| 引用本文： | 房保海,贾俊涛,梁旭锋,姜英辉,李正义,房倩,金莹.基于量子点的赭曲霉毒素AsFLISA检测技术研究[J].山东农业科学,2014(4):102-105. |
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| 作者姓名： | 房保海 贾俊涛 梁旭锋 姜英辉 李正义 房倩 金莹 |
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| 作者单位： | 山东出入境检验检疫局检验检疫技术中心;黄岛出入境检验检疫局; |
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| 基金项目： | 国家质检总局科技项目(2011IK221) |
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| 摘 要： | 建立了基于量子点标记技术的赭曲霉毒素A(Ochratoxin A,OTA)双抗夹心荧光免疫检测(sandwich fluorescence-linked immunosorbent assay,sFLISA)体系,包含多克隆包被抗体、生物素标记的多克隆检测抗体、量子点标记的链霉亲和素等,获得了sFLISA的最佳操作参数:包被抗体浓度2.5μg/mL,检测抗体稀释500倍,量子点标记链霉亲和素稀释100倍。该方法在OTA浓度3.125~125μg/L之间时,相对荧光强度和OTA浓度呈线性关系,回归方程为y=0.0206x+0.2018,R2=0.9924;加标回收率在90.1%~110.0%之间,变异系数均小于10%,能较好地进行赭曲霉毒素A的定量检测。
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| 关 键 词： | 赭曲霉毒素A（OTA） 量子点 双抗夹心荧光免疫检测（sFLISA） |
Study on sFLISA of Ochratoxin A Based on Quantum Dots Technique |
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| Fang Baohai,Jia Juntao,Liang Xufeng,Jiang Yinghui,Li Zhengyi,Fang Qian,Jin Ying.Study on sFLISA of Ochratoxin A Based on Quantum Dots Technique[J].Shandong Agricultural Sciences,2014(4):102-105. |
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| Authors: | Fang Baohai Jia Juntao Liang Xufeng Jiang Yinghui Li Zhengyi Fang Qian Jin Ying |
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| Institution: | 1. Inspection and Quarantine Technical Center of Shandong Exit ＆ Entry Inspection and Quarantine Bureau, Qingdao 266002, China;2. Huangdao Exit ＆ Entry Inspection and Quarantine Bureau, Qingdao 266555, China) |
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| Abstract: | The sandwich fluorescence -linked immunosorbent assay （ sFLISA） system was established to detect ochratoxin A （OTA）, which included coated antibody , detecting antibody （labeled by biotin）, strepta-vidin （ labeled by QDs ） and other elements .The optimal operating parameters were obtained：coated concen-tration was 2.5 μg/mL;dilution ratio of detecting antibody was 1∶500;dilution ratio of streptavidin （labeled by QDs） is 1∶100.There was a linear relationship between RFU and OTA concentration in the range of 3.125~125 μg/L, and the linear equation was y =0.0206x ＋0.2018 （R2 =0.9924）.The average recovery was between 90 .1%and 110 .0%, and the coefficient of variation was below 10%.This system could detect OTA correctly. |
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| Keywords: | Ochratoxin A （OTA） Quantum dots （QDs） Sandwich fluorescence -linked immunosor- bent assay （sFLISA） |
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