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| 转Bt基因玉米叶片中Bt蛋白的表达和分泌研究 | |
| 引用本文： | 田晓燕,赵蕾,孙红炜,李凡,赵辉,颜世磊,路兴波.转Bt基因玉米叶片中Bt蛋白的表达和分泌研究[J].山东农业科学,2012,44(1):11-13. |
| 作者姓名： | 田晓燕 赵蕾 孙红炜 李凡 赵辉 颜世磊 路兴波 |
| 作者单位： | 1. 山东师范大学生命科学学院,山东济南250014;山东省农业科学院植物保护研究所,山东济南250100 2. 山东师范大学生命科学学院,山东济南,250014 3. 山东省农业科学院植物保护研究所,山东济南,250100 |
| 基金项目： | 农业部转基因生物新品种培育重大专项 |
| 摘 要： | 以转Bt玉米Mon810及其亲本非转基因玉米为研究对象,通过酶联免疫吸附测定法(ELISA)定量检测Mon810玉米叶中Bt蛋白表达量和叶表Bt蛋白分泌量,结果表明叶组织和叶表均可检测到Bt蛋白,组织内Bt蛋白量从苗期到抽丝期随着植株营养生长的加快呈上升趋势,至抽丝期达到顶峰,之后逐渐降低;叶表Bt蛋白分泌量变化无明显规律性。 |
| 关 键 词： | 酶联免疫吸附测定法(ELISA) 转基因玉米 Bt蛋白 |
Study on Expression and Exudation of Bt Protein in Leaves of Transgenic Insect- resistant Corn |
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| TIAN Xiao-yan , ZHAO Lei , SUN Hong-wei , LI Fan , ZHAO Hui , YAN Shi-lei , LU Xing-bo.Study on Expression and Exudation of Bt Protein in Leaves of Transgenic Insect- resistant Corn[J].Shandong Agricultural Sciences,2012,44(1):11-13. | |
| Authors: | TIAN Xiao-yan ZHAO Lei SUN Hong-wei LI Fan ZHAO Hui YAN Shi-lei LU Xing-bo |
| Institution: | 1.College of Life Science,Shandong Normal University,Jinan 250014,China; 2.Institute of Plant Protection,Shandong Academy of Agricultural Sciences,Jinan 250100,China) |
| Abstract: | With the transgenic insect-resistant corn Mon810 and its non-transgenic parent as materials,the enzyme-linked immunosorbent assay(ELISA) method was used to detect the expression and quantitate the exudation of Bt protein in corn leaves.The results showed that Bt protein could be detected in tissue and on surface of leaves.The Bt content in tissues showed a rising trend from seedling stage to silking stage with the advances of vegetable growth,and reached the peak at the silking stage and then declined gradually.The variation of Bt exudation on leaf surface had no obvious regularity. |
| Keywords: | ELISA Transgenic corn Bt protein |
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