一种番茄根结线虫人工培养和保存的优化方法

根结线虫病在世界范围内对粮食蔬菜水果产量影响严重,其人工培养具有依赖寄主植物的局限性。从山东省泰安市房村镇的日光温室中采集被根结线虫侵染严重的番茄根系,用机械捣碎-过筛喷淋法从根系样品中分离根结线虫和卵,以牡丹根腐病镰刀菌(Fusarium solani)制作二重培养基,分别接种根结线虫悬液与卵悬液培养。结果显示,接种线虫悬液与卵悬液的平板分别在第三周与第四周线虫密度达到最高峰,培养基中虫口密度分别为2 300头/ml与2 000头/ml。置培养平板于15℃条件下存放,5个月后培养基内仍有可供继代繁殖的活虫体。该优化后的方法脱离了寄主植物束缚,简化了培养过程,具有高效繁殖和长期保存根结线虫的优点。

An Optimized Artificial Culture and Preservation Method for Tomato Root-knot Nematodes

Root-knot nematodes seriously decreased the production of crops,fruits and vegetables worldwide,and its artificial culture relied heavily on host plants.In this study,the tomato roots infected by root-knot nematodes were adopted from greenhouse of Fangcun Town,Taian City,Shandong Province,and the root-knot nematodes and eggs were separated by the method of mechanical beating-sifting spray and inoculated to double medium of Fusarium solani.The density of root-knot nematodes was about 2 300 per mini liter medium when inoculated with root-knot nematodes for three weeks and about 2 000 when inoculated with eggs for four weeks.Live root-knot nematodes could still be obtained after 5 months,if the culture plate was preserved at 15℃.This optimized method was independent of host plants,processed more simply,could obtain large quantity of root-knot nematodes in short time and preserve for long time.