| 应用RT—PCR检测辣椒上的黄瓜花叶病毒 |
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| 引用本文： | 杨超,孙秀东.应用RT—PCR检测辣椒上的黄瓜花叶病毒[J].山东农业科学,2009(2):94-96. |
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| 作者姓名： | 杨超 孙秀东 |
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| 作者单位： | 山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,山东,泰安,271018 |
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| 基金项目： | 山东农业大学博士科研基金，作物生物学国家重点实验室开放基金 |
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| 摘 要： | 根据黄瓜花叶病毒（CMV）的外壳蛋白基因序列设计、合成引物，运用两步法RT—PCR与一步法RT—PCR都可以从感病组织中扩增出与预期的780bp大小一致的目标片段。两种方法相比，一步法的特异性强、灵敏度高、省时经济、程序简单，达到了快速检测的目的。应用一步法RT—PCR对采集于山东辣椒产区的病毒病样本进行检测，结果表明6个采样地点的样本中均检测到CMV，在所采集的30个病毒病样本中CMV所占的比例为33．3％，说明CMV是山东省辣椒病毒病主要毒源之一。
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| 关 键 词： | RT—PCR 黄瓜花叶病毒 检测 |
Detection of CMV on Hot Pepper by RT-PCR |
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| YANG Chao,SUN Xiu-dong.Detection of CMV on Hot Pepper by RT-PCR[J].Shandong Agricultural Sciences,2009(2):94-96. |
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| Authors: | YANG Chao SUN Xiu-dong |
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| Institution: | College of Horticulture Science and Engineering of Shandong Agricultural University/ State Key Laboratory of Crop Biology;Taian 271018;China |
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| Abstract: | A pair of specific primers was designed and synthesized based on the nucleotide sequence of CMV coat protein genes reported before.The expected product(780 bp DNA fragment) was gained by one-step RT-PCR and two-step RT-PCR from the infected samples.Compared with two-step RT-PCR,one-step RT-PCR showed strong specificity,high sensitivity,simple procedure,economical and saving time,so it could meet short-term test.Thirty pepper samples with virus symptom collected from Shandong area were tested by one-step RT-... |
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| Keywords: | RT-PCR |
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